Sequences from confirmation amplicon


Confirmation sequences for line 476D03 and hit At3g55730 are shown.
Line ID 476D03
Locus At3g55730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20683322
Primers used for PCR RS12 - 8474, show primer details
   
Sequence Name 15-K019984-022-476-D03C-RS12
GenBank Accession LN492767 [GenBank]
Sequencing primer gene specific primer, RS12: 5'-GAGGAGGAGGGATTAGGAGTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K019984-022-476-D03C-RS12
CTTGGTCTACGGAGGAAGACGCGGTTCTTACGAAGCTTGTGAGGAAACTAGGACCGAGGA
ATTGGAGTTTGATCGCTCGTGGAATCCCTGGTCGTTCTGGTAAATCTTGTCGATTGCGTT
GGTGTAATCAGCTTGACCCTTGTCTCAAGCGTAAACCTTTCTCTGGTAAACAAACTTTAA
AACCCTCTTGATTTTGTTACATCTAAATTCAGATTCTTTGCTTTTGGGTTTTGGGTTTTT
GAAGTTTCGTTTGAATTGAATTTACTGTTC
   
Sequence Name 16-K019984-022-476-D03C-8409
GenBank Accession LN493236 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K019984-022-476-D03C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAACGTTTTTTTGGTCTGTAG
AATCAGAGTAATTGTTATTACATTGTTATTACTTACAATGTTTAATCCATTGACATTACA
TTTACAGAGTAATCAGAGTAATAACAATAAAACAGTCAAACATTGTTCTCATTCACTTCA
CAATCTTCTAACCACAGTAGAATCAGAATTACACAAAACAAAGGAAAACCCAGTAATTTG
ACACACAAGTCAAAAAATTCATTTGAAGACGAGAACAAACAAAACAAACCACTCAAGAAT
TTGTAATGAAGATCGAGAAAATCACTCTGAACATTTGCAAGAAGAACAGTAAAATCAAAT
CAAACGAAACCTCAAAAACCCAAAACCCAAAAGCAAAGAATCTGAATTTAGATGTAACAA
AATCAAGAGGGTTTTAAAGTTTGTTTACCAGAGAAAGGTTTACGCTTGAGACAAGGGTCA
AGCTGATTACACCAACGCAATCGACAAGATTTACCAGAACGACCAGGGATTCCACGAGCG
ATCAAACTCCAATTCCTCGGTCCTAGTTTCCTCACAAGCTTCGTAAGAACCGCGTCTTCC
TCCGTAGACCAAGGTCCTTTGACTTTACTCCT

Last Updated on 06.12.2018 16:11