Sequences from confirmation amplicon


Confirmation sequences for line 477E05 and hit At5g51710 are shown.
Line ID 477E05
Locus At5g51710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21006077
Primers used for PCR TH10 - 8474, show primer details
   
Sequence Name 47-K103582-0022-477-E05C-TH10
GenBank Accession LN506177 [GenBank]
Sequencing primer gene specific primer, TH10: 5'-AAACAGATTACGAATTGGTTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103582-0022-477-E05C-TH10
ACCACCTTAAGACTTCATTTTGTACCACGTACTCGCAAAGCAAAAGCGACCTGAGTTTAG
AATCAATACCTGCTCTAGTGTATGTTGAGCAAATTCAGTCGTAGACAACATAACGCCAGC
CACAAATGATCCCAACTCAAGACTAAGACCCAGCTTGTCACTGCACTGAAAAAGTTTTGC
GAGGAACCAACACTCTAAGTTAAAAATTGGAAATTACATCAATATGCTATTATACTTAAA
TACTTCAGTGTCTATTGTAATCATCATTTTTTTCTTCTGTAGTCTATTAGTAAGATGCAA
GCTCAGGTAACAATTAACTCAGATGTTATAATACACCAAAAGGGATCAGAAAGCTAAGCA
AGCACCAGTATGCAAAGAAATGACAGAATTAGACTTACCCATGCAGATAGTAAGCAGAAT
GCCACAGCAGCTAACTGGTAAAGTTCATTTGTCTGCATTATATAATGAGAGACATTGGCA
ATAATTTGGATCCCACTTTAAGAAAGAAATAAAGATAGACAAGTAGTGTATATGAAGATA
TGACGTCTAAAACGTACTTGAGATGATAATTGAATCATTAGCTTCAGAAAACGAGGTACA
AATGACCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGT
   
Sequence Name 63-K103583-0022-477-E05C-8409
GenBank Accession LN510969 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103583-0022-477-E05C-8409
CCTGAAGGTCATTTGTACCTCGNTTTTCTGAAGCTAATGATTCAATTATCATCTCAAGTA
CGTTTTAGACGTCATATCTTCATATACACTACTTGTCTATCTTTATTTCTTTCTTAAAGT
GGGATCCAAATTATTGCCAATGTCTCTCATTATATAATGCAGACAAATGAACTTTACCAG
TTAGCTGCTGTGGCATTCTGCTTACTATCTGCATGGGTAAGTCTAATTCTGTCATTTCTT
TGCATACTGGTGCTTGCTTAGCTTTCTGATCCCTTTTGGTGTATTATAACATCTGAGTTA
ATTGTTACCTGAGCTTGCATCTTACTAATAGACTACAGAAGAAAAAAATGATGATTACAA
TAGACACTGAAGTATTTAAGTATAATAGCATATTGATGTAATTTCCAATTTTTAACTTAG
AGTGTTGGTTCCTCGCAAAACTTTTTCAGTGCAGTGACAAGCTGGGTCTTAGTCTTGAGT
TGGGATCATTTGTGGCTGGCGTTATGTTGTCTACGACTGAATTTGCTCAACATACACTAG
AGCAGGTATTGATTCTAAACTCAGGTCGCTTTTGCTTTGCGAGTACGTGGTACAAAATGA
AGTCTTAAGGTGGTGGAATCTCCCTTACTTTGTGATGTAGGTGGAACC

Last Updated on 06.12.2018 16:11