Sequences from confirmation amplicon


Confirmation sequences for line 477H04 and hit At1g13050 are shown.
Line ID 477H04
Locus At1g13050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4451392
Primers used for PCR 49kq - 8474, show primer details
   
Sequence Name 49-K113595-0022-477-H04C-49kq
GenBank Accession LN506908 [GenBank]
Sequencing primer gene specific primer, 49kq: 5'-ATTCTCCTCTTAGTCTACCTCGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113595-0022-477-H04C-49kq
CTCAGCAGCACCCTAAACACCGCCATCTGGACATGGGATATGTTCTAAATGGAGATCTCG
CGGTTGTGGTAAACTTCACAAATCCAAGCAAGAAAAGCAGTGTGGACTTCAGCTACGTGA
TGTTCGAGCTCTACTTCTACAACACACTCATAGCGACTGAACACATCGAGCCATTCATTG
TACCAAAGGGAATGTCAATGTTCACAAGCTTCCATCTCGTCAGCAGTCAGGTACAAATTC
AGATGATTCAGAGTCAGGACTTGCAGCTGCAGCTCGGAACTGGTCCAGTGTTGTTGAATT
TGAGAGGAACGTTTCACGCACAGTGCAGCATCTCTTATTGGTCGTATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTC
   
Sequence Name 07-K113594-0022-477-H04C-8409
GenBank Accession LN489355 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113594-0022-477-H04C-8409
CGTTCCTCTCAATTCAACAACACTGGACCAGTTCCGAGCTGCAGCTGCAAGTCCTGACTC
TGAATCATCTGAATTTGTACCTGACTGCTGACGAGATGGAAGCTTGTGAACATTGACATT
CCCTTTGGTACAATGAATGGCTCGATGTGTTCAGTCGCTATGAGTGTGTTGTAGAAGTAG
AGCTCGAACATCACGTAGCTGAAGTCCACACTGCTTTTCTTGCTTGGATTTGTGAAGTTT
ACCACAACCGCGAGATCTCCATTTAGAACATATCCCATGTCCAGATTGGCGGTGTTTAGG
GTTGCTGCTGAGATGTCGAAGTATGGTGAGCGTGGACGGTTGGCGAGGTAGACTAGAANG
AGAA

Last Updated on 06.12.2018 16:11