Sequences from confirmation amplicon


Confirmation sequences for line 478A02 and hit At1g50280 are shown.
Line ID 478A02
Locus At1g50280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18624627
Primers used for PCR 33cy - 8474, show primer details
   
Sequence Name 55-K109676-0022-478-A02C-33cy
GenBank Accession LN508840 [GenBank]
Sequencing primer gene specific primer, 33cy: 5'-TCGACATATCTCTGTTCGATCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K109676-0022-478-A02C-33cy
ATAACAAACAACAGAAATTATAAAGGAATCAATCATACACTGACATAATAATCATTATTT
CTACAATTTTTGAAAATGATGAACTATTGTTAATTCCAGTAAAACATGTGAATAGAAAAG
TACATGAATAATTGTTAGTCTTTGACATGTATTTCAAGTGTAACTGATAGTACCTTACCA
GAATTATTCCTTTTTAAGATATTTTTACTAATAGTATTACAGAGAAAAACAAGAAGAAAC
ATACAGTCAAGACTGTTGTGGCCTTGTGGGTCTGACATGAACCCCACGGCAGTAACAAGA
CAGAGTGAAGAATCTAAAGGATGACTCCAAATCTTCTTAATCATATATATACATCCATGT
GTGTATATTATTAATACACGTTAATCTCAAAAAACTTCAAATCTTTTCTTTGATACATTT
TCGGTTTTATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 39-K109675-0022-478-A02C-8409
GenBank Accession LN503383 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K109675-0022-478-A02C-8409
GCCATTTACAATTGAATATATCCTGAAATAAAACCGAAAATGTATCAAAGAAAAGATTTG
AAGTTTTTTGAGATTAACGTGTATTAATAATATACACACATGGATGTATATATATGATTA
AGAAGATTTGGAGTCATCCTTTAGATTCTTCACTCTGTCTTGTTACTGCCGTGGGGTTCA
TGTCAGACCCACAAGGCCACAACAGTCTTGACTGTATGTTTCTTCTTGTTTTTCTCTGTA
ATACTATTAGTAAAAATATCTTAAAAAGGAATAATTCTGGTAAGGTACTATCAGTTACAC
TTGAAATACATGTCAAAGACTAACAATTATTCATGTACTTTTCTATTCACATGTTTTACT
GGAATTAACAATAGTTCATCATTTTCAAAAATTGTAGAAATAATGATTATTATGTCAGTG
TATGATTGATTCCTTTATAATTTCTGTTGTTTTGTTATTGTACAGTCTCATCCGAATCTA
ACACCCCAAGATCGAACAGAGATATGTCGA

Last Updated on 06.12.2018 16:11