SimpleSearch - Line and FST details


Line specific information

 
Line ID 478A02
Vector Used pAC161
Line Availability available as T3 set from NASC (N445794)
Segregation Analysis 50:8:7
Confirmed for Hit At1g50280
Parent of DUPLO pair 2500
Parent of pair(s) none
Note

Gene hit At1g50280

 
Sequence (A. th genome BLAST matches underlined) 
>09-K026075-022-478-A02-8409
AACCATTTACATTGAATATATCCTGAAATAAAACCGAAAATGTATCAAAGAAAAGATTTG
AAGTTTTTTGAGATTAACGTGTATTAATAATATACACACATGGATGTATATATATGATTA
AGAAGATTTGGAGTCATCCTTTAGATTCTTCACTCTGTCTTGTTACTGCCGTGGGGTTCA
TGTCAGACCCACAAGGCCACAACAGTCTTGACTGTATGTTTCTTCTTGTTTTTCTCTGTA
ATACTATTAGTAAAAATATCTTAAAAAGGAATAATTCTGGTAAGGTATGGGATCCTCCCT
ATAGTGAGANNNNNNNNNNNNNNNNNNNNNNNNANNNNGGGACTATGGGATCCCTTTCAT
TTTATAATAAGGCTGCGGACAT
GenBank Accession CR397504 [GenBank]
Graphic View Graphic view of gene At1g50280
Predicted Position of Insertion Chr1:18624627 - go to primer design
BLAST e Value 3e-145
Hit Clone Code (BAC ID) F14I3
Hit Gene Code At1g50280 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Phototropic-responsive NPH3 family protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit CR397503 [GenBank]

Gene hit At5g51710

 
Sequence (A. th genome BLAST matches underlined) 
>09-K019033-022-478-A02-8409
AGGTAGTGATTACTTGATATCGTACGATATTACTAAAAATAAAGAGTTGAGAAAATAAAA
AAAGCAAGTCTTGGATCTGATTTGGTCAATTTCAAATGTTTTTTCTGCCTCATATGGGAT
CCTCCCTATAGAGAAACCGACGCACCGGGCAGGCGGATCCATGCTTTCCAACGAAGCACG
CCCATCCAACCTATGGGATCCTCCCTATAGTGAG
GenBank Accession BX293392 [GenBank]
Graphic View Graphic view of gene At5g51710
Predicted Position of Insertion Chr5:21004313 - go to primer design
BLAST e Value 3e-23
Hit Clone Code (BAC ID) K10D11
Hit Gene Code At5g51710 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation K efflux antiporter 5
Insertion Classification TS2TE (3')
Confirmation Status failed, line belongs to a contamination group, please check line 397G01


Last Updated on 10.06.2021 13:37