Sequences from confirmation amplicon


Confirmation sequences for line 478H10 and hit At1g67280 are shown.
Line ID 478H10
Locus At1g67280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25189860
Primers used for PCR 17wu - 8474, show primer details
   
Sequence Name 42-K118761-0022-478-H10C-17wu
GenBank Accession LN504646 [GenBank]
Sequencing primer gene specific primer, 17wu: 5'-AGATGTTCCTCAATCACAGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118761-0022-478-H10C-17wu
GTAAACTGTTTAGGAGTTGCTGAATCTGGTAAAGCAGCTCAAGCTACTACACAAGATGAT
CTTCTCACCTGGGTTAAGAATGATAAGAGAAGAATGCTTCATGTTGTTTATCGTGTTGGC
GATATGGACCGGACGATAAAGTACTTCACTTTGGCTTTCCTGTGATATGGCTTTGATGTT
GAGTCTCTTAAAGTCACATTAAGTTTAAAAGAATCATCTGATTGCTGTAGGTTTTATACT
GAATGTCTTGGGATGAAGCTTCTACGTAAGCGTGATATACCAGAAGAGAAGTATACTAAT
GCTTTCCTTGGTTATGGACCGGAAGATTCACACTTTGTGATTGAGCTTACTTATAGTAAG
TTATATCTACCAAATTTCCAATTATTAAAATTACATGCATTTGGTAAGTTATATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
GCACAGTTATT
   
Sequence Name 17-K118760-0022-478-H10C-8409
GenBank Accession LN494073 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118760-0022-478-H10C-8409
AATTGAATAATATAACTTACCAAATGCATGTAATTTTAATAATTGGAAATTTGGTAGATA
TAACTTACTATAAGTAAGCTCAATCACAAAGTGTGAATCTTCCGGTCCATAACCAAGGAA
AGCATTAGTATACTTCTCTTCTGGTATATCACGCTTACGTAGAAGCTTCATCCCAAGACA
TTCAGTATAAAACCTACAGCAATCAGATGATTCTTTTAAACTTAATGTGACTTTAAGAGA
CTCAACATCAAAGCCATATCACAGGAAAGCCAAAGTGAAGTACTTTATCGTCCGGTCCAT
ATCGCCAACACGATAAACAACATGAAGCATTCTTCTCTTATCATTCTTAACCCAGGTGAG
AAGATCATCTTGTGTAGTAGCTTGAGCTGCTTTACCAGATTCAGCAACTCCTAAACAGTT
TACACTTCTTCTCAAAAGCTTATGAGAAGTTAAACCAAAAAGCTGTGATTGAGGAACATC

Last Updated on 06.12.2018 16:11