Sequences from confirmation amplicon


Confirmation sequences for line 479B03 and hit At5g41400 are shown.
Line ID 479B03
Locus At5g41400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16569654
Primers used for PCR HF49 - 8474, show primer details
   
Sequence Name 35-K101326-0022-479-B03C-8409
GenBank Accession LN501762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101326-0022-479-B03C-8409
GAGCCTTTACAATTGAATATATCCTGAAATAAACGGCGTTCTACAAAGTGGACACGTCAT
CTGATTATAACCCATCATCCAACGGTCTAAACAGCTCCGGTGAAATATGTGTTGACAATT
CGTCAGCCGTCGGATCTCGTCATCGTTCTCGAACTCGTGGAGGCACACCGCGCAGCAATC
GGATCCGGATCCGAATCCGGGTCGAGTTAGCTCCGAGAATCTGATGACGGGCAAGATTTC
TCCAGCTAGCCTCGCCGCCACTGGGAAGAAAAATGAGCTTTCTTGGTGATGATGCGCCGC
CGTGGACATATACGGTGGTTCCAGCCATGACGATGACGATGAAACCGGGTCGGATTCGAG
AAAGTCGGGTAGACCCAATATCCGAAAACCCGTGTCTATGAGTGTTCGTATTAAGCCTAA
GAGAGAGAGTAAGTGAAGGAAGATTCTTGGGAGGAGGAGCTCAGTGTACCCCACCGGATA
ACCCATGGCTTCGCCGCTNGAAAGAGAAAGCTAAAGAGATNGAGAGAGAGCGCG
   
Sequence Name 35-K101332-0022-479-B03C-HF49
GenBank Accession LN501763 [GenBank]
Sequencing primer gene specific primer, HF49: 5'-CGCGCTCTCTCTCTATCTCTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101332-0022-479-B03C-HF49
GGTGGGGTACACTGAGCTCCTCCTCCCAGAATCTTCCCTTAATACTCTCTCTCTTAGGCT
TAATACGAACACTCATACACACGGGTTTTCGGATATTGGGTCTACCCGACTTTCTCGAAT
CCGACCCGGTTTCATCGTCATCGTCATGGCTGGAACCACCGTATATGTCCACGGCGGCGC
ATCATCACCAAGAAAGCTCATTTTTCTTCCCAGTGGCGGCGAGGCTAGCTGGAGAAATCT
TGCCCGTCATCAGATTCTCGGAGCTAACTCGACCCGGATTCGGATCCGGATCCGATTGCT
GCGCGGTGTGCCTCCACGAGTTCGAGAACGATGACGAGATCCGACGGCTGACGAATTGTC
AACACATATNTCACCGGAGCTGTTTAGACCGTTGGATGATGGGTTATAATCAGATGACGT
GTCCACTTTGTAGAACGCCGTTTATTTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11