Genotyping and primer details

Genotyping and primer details for 479B03 - At5g41400
Line ID 479B03
Locus At5g41400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16569654 of line 479B03 Chr5:16569654
Insertion allele amplicon 8474 - HF49
Locus specific primer HF49: 5'-CGCGCTCTCTCTCTATCTCTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16570162
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11