Sequences from confirmation amplicon


Confirmation sequences for line 480A12 and hit At3g10550 are shown.
Line ID 480A12
Locus At3g10550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3294394
Primers used for PCR 66qz - 8474, show primer details
   
Sequence Name 72-K115869-0022-480-A12C-66qz
GenBank Accession LN512783 [GenBank]
Sequencing primer gene specific primer, 66qz: 5'-AAAATCTACCAGGAAAGTCTGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K115869-0022-480-A12C-66qz
CTGGAAGAAGTTGAACATGAAATCTTCAGAAATATGTTTGACTTCATACATACCGGAGAA
AACTCAAAAGCACAAGGATACATCCGCATTAGCTGTGAAACGCTATCAATCCACTGGAAA
CACATAGATGTAAACATGGATCAGCAATGAGTATTACTATAATTGCAATATTCAATTGCA
AGATCTAAACATGGATCATATACTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 31-K115867-0022-480-A12C-8409
GenBank Accession LN500300 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K115867-0022-480-A12C-8409
TACATTGAATATATCCTGACAGTATATGATCCATGTTTAGATCTTGCAATTGAATATTGC
AATTATAGTAATACTCATTGCTGATCCATGTTTACATCTATGTGTTTCCAGTGGATTGAT
AGCGTTTCACAGCTAATGCGGATGTATCCTTGTGCTTTTGAGTTTTCTCCGGTATGTATG
AAGTCAAACATATTTCTGAAGATTTCATGTTCAACTTCTTCCAGTAATAATAGTGTAGTC
ATTTGCCTTCATATTTGGTGTTGCAGACTTTCCGGNTAGATTTTA

Last Updated on 06.12.2018 16:11