Sequences from confirmation amplicon


Confirmation sequences for line 480D08 and hit At2g43040 are shown.
Line ID 480D08
Locus At2g43040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17896940
Primers used for PCR 21zd - 8474, show primer details
   
Sequence Name 29-K123937-0022-480-D08C-21zd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21zd: 5'-AAGAAGTAATGCCTGGTGTGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K123937-0022-480-D08C-21zd
GCTCTTTCTTATGTGCAGCAGGTCAAAACAGTGCTGCAGTTAACCTTCTTAGAAAGTCTC
TGCATAAACACGAACAACCCGATGATCTTGTGGCGCTTTTGTTAGCTGCTAAGCTTTGCA
GTGAAGAGCCTTCTTTAGCTGCTGAAGGTACGGGTTATGCGCAGAGAGCGATAAACAATG
CTCAAGGTATGGATGAGCATTTGAAAGGCGTTGGTTTGAGGATGTTAGGACTTTGTTTAG
GGAAACAAGCGAAGGTTCCGACATCGGATTTTGAAAGATCTCGGCTGCAATCAGAATCAT
TGAAAGCATTAGATGGAGCTATAGCTTTTGAGCACAATAATCCTGATTTGATCTTTGAGT
TAGGTGTTCAATACGCTGAGCAACGGAACTTAAAAGCTGCTTCCCGTTACGCCAAAGAGT
TCATCGATGCAACGGGAGGGTCAGTGTTAAAAGGATGTAGCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGC
   
Sequence Name 13-K123936-0022-480-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K123936-0022-480-D08C-8409
TTGAATATATCCTGCTACATCCTTTTAACACTGACCCTCCCGTTGCATCGATGAACTCTT
TGGCGTAACGGGAAGCAGCTTTTAAGTTCCGTTGCTCAGCGTATTGAACACCTAACTCAA
AGATCAAATCAGGATTATTGTGCTCAAAAGCTATAGCTCCATCTAATGCTTTCAATGATT
CTGATTGCAGCCGAGATCTTTCAAAATCCGATGTCGGAACCTTCGCTTGTTTCCCTAAAC
AAAGTCCTAACATCCTCAAACCAACGCCTTTCAAATGCTCATCCATACCTTGAGCATTGT
TTATCGCTCTCTGCGCATAACCCGTACCTTCAGCAGCTAAAGAAGGCTCTTCACTGCAAA
GCTTAGCAGCTAACAAAAGCGCCACAAGATCATCGGGTTGTTCGTGTTTATGCAGAGACT
TTCTAAGAAGGTTAACTGCAGCACTGTTTTGACCTGCTGCACTATAAGAAAGAGCCAAAG
TGTTCCAACGCTCAATACGGCTAAACACACCAGGATTTACTTCT

Last Updated on 06.12.2018 16:11