Sequences from confirmation amplicon


Confirmation sequences for line 480G06 and hit At4g27720 are shown.
Line ID 480G06
Locus At4g27720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13832374
Primers used for PCR 12um - 8474, show primer details
   
Sequence Name 42-K117317-0022-480-G06C-12um
GenBank Accession LN504613 [GenBank]
Sequencing primer gene specific primer, 12um: 5'-CATTGCTACTTCCCTTCTGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117317-0022-480-G06C-12um
GGTAAAGAAAGGTTTATGCTGTATTAAGCTCTTAAGCTGCCATGTTTATTTATAAACTTA
GTGTTTTTATTCGTGATGTATGCAGAGGGGCTTTGAGCAGCAATGGCTGTCTGTGACATT
TTCAAAGGCAGTGTTTTTTGGCAACGGTCTCGTTGCAATTATCGCTGGATTGTTTGGGAA
CCTGCTTGTTGATACCTTCTCTCTTGGGCCTGTTGCGCCATTTGACGCTGCTGCATGCTT
TCTTACTATTGGAATGGCTGTCATTCTATCATCATGGACAGAAAACTATGGAGATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACAGTTATTAT
   
Sequence Name 33-K117316-0022-480-G06C-8409
GenBank Accession LN501159 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117316-0022-480-G06C-8409
TTGAATATCTCCATAGTTTTCTGTCCATGATGATAGAATGACAGCCATTCCAATAGTAAG
AAAGCATGCAGCAGCGTCAAATGGCGCAACAGGCCCAAGAGAGAAGGTATCAACAAGCAG
GTTCCCAAACAATCCAGCGATAATTGCAACGAGACCGTTGCCAAAAAACACTGCCTTTGA
AAATGTCACAGACAGCCATTGCTGCTCAAAGCCCCTCTGCATACATCACGAATAAAAACA
CTAAGTTTATAAATAAACATGGCAGCTTAAGAGCTTAATACAGCATAAACCTTTCTTTAC
CTTATTGTGCTCAGCAACTAGCCATGATTCAAATGACGAAAACAGAAGGGAGNTAGCAAT
G

Last Updated on 06.12.2018 16:11