Sequences from confirmation amplicon


Confirmation sequences for line 480G12 and hit At5g10100 are shown.
Line ID 480G12
Locus At5g10100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3158010
Primers used for PCR JV57 - 8474, show primer details
   
Sequence Name 14-K101324-0022-480-G12C-8409
GenBank Accession LN492437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101324-0022-480-G12C-8409
TTCGGACTGTGAGACTACATGTCAAGTATCATCCCCAACAACAACAACAACAACAACAAC
TCTTCTTCACAGAAACTCCCTCCTTGTTTAATCTCAATTTCCAAGAAAAAGCTTCTCAAG
AACATCGACATCATCAATGGTGGTGGACAAAGAATCAACGCTTGGGTAGATTCAATGCGT
GCTTCTTCTCCTACTCATCTCAAATCTCTTCCTTCTTCTATCTCCACACAGCAACAACTC
AACTCATGGATCGTAAGTGTTTTCAATAGTCTCTCACAAACTCCTGATAAGTCTTCCTCT
TACATTTTTTCTTTCTTTCTTGTGTGTTTTACGACAGATGCAACATCCTTCAGCACTAGA
AAAATTCGAACAGATAATGGAAGCTTCGAGAGGGAAACAAATCGTAATGTTTCTTGATTA
TGACGGTACTCTCTCTCCCA
   
Sequence Name 14-K101341-0022-480-G12C-JV57
GenBank Accession LN492438 [GenBank]
Sequencing primer gene specific primer, JV57: 5'-AATGGGAGAGAGAGTACCGTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101341-0022-480-G12C-JV57
ATTTGTTTCCTCTCGAGCTTCCATTATCTGTTCGAATTTTTCTAGTGCTGAAGGATGTTG
CATCTGTCGTAAAACACACAAGAAAGAAAGAAAAAATGTAAGAGGAAGACTTATCAGGAG
TTTGTGAGAGACTATTGAAAACACTTACGATCCATGAGTTGAGTTGTTGCTGTGTGGAGA
TAGAAGAAGGAAGAGATTTGAGATGAGTAGGAGAAGAAGCACGCATTGAATCTACCCAAG
CGTTGATTCTTTGTCCACCACCATTGATGATGTCGATGTTCTTGAGAAGCTTTTTCTTGG
AAATTGAGATTAAACAAGGAGGGAGTTTCTGTGAAGAAGAGTTGTTGTTGTTGTTGTTGT
TGTTGGGGATGATACTTGACATTGTAGTCTCTACATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11