Sequences from confirmation amplicon


Confirmation sequences for line 481D05 and hit At3g03180 are shown.
Line ID 481D05
Locus At3g03180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:734329
Primers used for PCR 11ie - 8474, show primer details
   
Sequence Name 39-K112875-0022-481-D05C-8409
GenBank Accession LN503411 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112875-0022-481-D05C-8409
ATAAGTCTAAATAGTAAAACAGAGAGAAGTTGCAATGAATGTACCGATCTGATGTATGGT
TGCTGCAAAAGCCAGCCTAATAGAGGTATCCTTTGAAGGAAGACGGCAAGTGTAGGCCAG
AAACCACTTTAAAATTACAGAAGAATAAAGGAAAGACATGAGTCAGCAAATCAAAACTAG
AAATGAATTAGTAAAACCATTAAATGTTGTTGAGGAGAAGAAAACCCACCTGAAGAGCAC
AAGGAACCCATATGATTCAAGAAGCAAACCGAAAATAGGCCATCCAAACACAACCAACAA
GAAACCCAATCCAAATGATATAGTACCCTGTATGTATATCAAGAAGATGTATTATTGTAT
TAATAATAGTCATCAAATTGGAAGCACAAGGGATTCATGAAAGAACCTTAAAATTCTGCC
GTTTAGTAAAGAATTGGATTGCTGGGTTGATTCCNATGGTTAGAGTCACACCAGCTAAGA
AGAGG
   
Sequence Name 55-K112876-0022-481-D05C-11ie
GenBank Accession LN508862 [GenBank]
Sequencing primer gene specific primer, 11ie: 5'-CCTCTTCTTAGCTGGTGTGACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K112876-0022-481-D05C-11ie
CGGCAGAATTTTAGGTTCTTTCATGAATCCCTTGTGCTTCCAATTTGATGACTATTATTA
ATACAATAATACATCTTCTTGATATACATACAGGGTACTATATCATTTGGATTGGGTTTC
TTGTTGGTTGTGTTTGGATGGCCTATTTTCGGTTTGCTTCTTGAATCATATGGGTTCCTT
GTGCTCTTCAGGTGGGTTTTCTTCTCCTCAACAACATTTAATGGTTTTACTAATTCATTT
CTAGTTTTGATTTGCTGACTCATGTCTTTCCTTTATTCTTCTGTAATTTTAAAGTGGTTT
CTGGCCTACACTTGCCGTCTTCCTTCAAAGGATACCTCTATTAGGCTGGCTTTTGCAGCA
ACCATACATCAGATCGGTACATTCATTGCAACTTCTCTCTGTTTTACTATTTAGACTTAT
TTGCCTTATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGATTATA

Last Updated on 06.12.2018 16:11