Sequences from confirmation amplicon


Confirmation sequences for line 481G03 and hit At3g18780 are shown.
Line ID 481G03
Locus At3g18780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6475806
Primers used for PCR SD23 - 8474, show primer details
   
Sequence Name 20-K103492-0022-481-G03C-SD23
GenBank Accession LN495176 [GenBank]
Sequencing primer gene specific primer, SD23: 5'-CCGATCAGTTAGCTCGATTATAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103492-0022-481-G03C-SD23
GAGAGATCCTGTTCATGTTACCTGGGAAATGATTTGTATATGTGAATTGAAATCTGAACT
GTTGAAGTTAGATTGAATCTGAACACTGTCAATGTTAGATTGAATCTGAACACTGTTTAA
GGTTAGATGAAGTTTGTGTATAGATTCTTCGAAACTTTAGGATTTGTAGTGTCGTACGTT
GAACAGAAAGCTATTTCTGATTCAATCAGGGTTTATTTGACTGTATTGAACTCTTTTTGT
GTGTTTGCAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACA
ATGGTACCGGTATGGTGAAGGCTGGATTTGCAGGAGATGATGCTCCCAGGGCTGTTTTTC
CCAGTGTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGGATGAACCAGAAGGATG
CATATGTTGGTGATGAAGCACAATCCAAGAGAGGTATTCTTACCTTGAAGTATCCTATTG
AGCATGGTGTTGTTAGCAACTGGGATGATATGGAAAAGATCTGGCATCACACAAATTTAC
ATGAGGCTCCTCTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 44-K103493-0022-481-G03C-8409
GenBank Accession LN505153 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103493-0022-481-G03C-8409
ATTTACAATTGAATATATCCTGTAGAGGAGCCTCATGTAAATTTGTGTGATGCCAGATCT
TTTCCATATCATCCCAGTTGCTAACAACACCATGCTCAATAGGATACTTCAAGGTAAGAA
TACCTCTCTTGGATTGTGCTTCATCACCAACATATGCATCCTTCTGGTTCATCCCAACCA
TGACACCATGATGTCTTGGCCTACCAACAACACTGGGAAAAACAGCCCTGGGAGCATCAT
CTCCTGCAAATCCAGCCTTCACCATACCGGTACCATTGTCACACACGATTGGTTGAATAT
CATCAGCCTCAGCCATTTTTTATGAGCTGCAAACACACAAAAAGAGTTCAATACAGTCAA
ATAAACCCTGATTGAATCAGAAATAGCTTTCTGTTCAACGTACGACACTACAAATCCTAA
AGTTTCGAAGAATCTATACACAAACTTCATCTAACCTTAAACAGTGTTCAGATTCAATCT
AACATTGACAGTGTTCAGATTCAATCTAACTTCAACAGTTCAGATTTCAATTCACATATA
CAAATCATTTCCCAGGTAACATGAACATGGATCTCTCCATCAAGGTCAAGCCAAATTCTG
GTAGCTATAATC

Last Updated on 06.12.2018 16:11