Sequences from confirmation amplicon


Confirmation sequences for line 484B11 and hit At5g11470 are shown.
Line ID 484B11
Locus At5g11470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3664055
Primers used for PCR AK49 - 8474, show primer details
   
Sequence Name 35-K105706-0022-484-B11C-AK49
GenBank Accession LN501835 [GenBank]
Sequencing primer gene specific primer, AK49: 5'-AAATTCAAGGTTGTTAGGCTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K105706-0022-484-B11C-AK49
CTCTGGAAACTTAAAGACCAAAGAGCAAGAAGATTAGTAACGTAACAATGCATTTTTATT
TGTGTGCATTATGCAAGGGAAAGGGAAATGAAAACAATACCATCTCCCGTCGCGTTTGAG
TTTTGTGTAGTTTGATATGGCCTGAGAATAATGACGGCTTCCCTGGTGGAGTAATCTTAG
CGAAAGAAGCAACAAGGGGCCTGTTGAGCAAAAAAGGCACAGCAGAGATTTCTGAGATGA
AAGTATGCCAAAAAAATGGCACTGTACATCTCTGCAATTTATCATAGGAAGCTTAGAGAA
AAAAGAAGAATATGTCATTTCAGGTTCACCTCCCACTTGATAGCAACAAGCATCCCTCAT
CTAGTCTTCTAATCACCCTTTCTGCAACTTCTCTTGTTTTGAAAATGACCAAAGCTTCAC
CTGTATATCAAACAATATGAGGTGCTCTTCATTCCATTTATAGTATGTGAGAATCATAAA
TTAGAACCCGGTGAGTAAAATGGTAGCTTACCAATATGAGGAATAGTGACCTTTTTCTTT
CTCTTCAAGGACTTAAAATACTAAGACCAGAAGACACATAANAGGAAAAAACCATCACAA
GATAGATCAAATCAGAAGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGAA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCANAAATGTAGATT
   
Sequence Name 51-K105707-0022-484-B11C-8409
GenBank Accession LN507503 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K105707-0022-484-B11C-8409
GCCATTTACATTGAATCTTCTGATTTGATCTATCTTGTGATGGTTTTTTCCTTTTATGTG
TCTTCTGGTCTTAGTATTTTAAGTCCTTGAAGAGAAAGAAAAAGGTCACTATTCCTCATA
TTGGTAAGCTACCATTTTACTCACCGGGTTCTAATTTATGATTCTCACATACTATAAATG
GAATGAAGAGCACCTCATATTGTTTGATATACAGGTGAAGCTTTGGTCATTTTCAAAACA
AGAGAAGTTGCAGAAAGGGTGATTAGAAGACTAGATGAGGGATGCTTGTTGCTATCAAGT
GGGAGGTGAACCTGAAATGACATATTCTTCTTTTTTCTCTAAGCTTCCTATGATAAATTG
CAGAGATGTACAGTGCCATTTTTTTGGCATACTTTCATCTCAGAAATCTCTGCTGTGCCT
TTTTTGCTCAACAGGCCCCTTGTTGCTTCTTTCGCTAAGATTACTCCACCAGGGAAGCCG
TCATTATTCTCAGGCCATATCAAACTACACAAAACTCAAACGCGACGGGAGATGGTATTG
TTTTCATTTCCCTTTCCCTTGCATAATGCACACAAATAAAAATGCATTGTTACGTTACTA
ATCTTCTTGCTCTTTGGTCTTTATATGTTTTTCCAGAGAGATGCCGTGGCTACATCACAT
TCTTCTCAGCC

Last Updated on 06.12.2018 16:11