Sequences from confirmation amplicon


Confirmation sequences for line 485E02 and hit At2g40925 are shown.
Line ID 485E02
Locus At2g40925 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17075899
Primers used for PCR CC58 - 8474, show primer details
   
Sequence Name 15-K106588-0022-485-E02C-8409
GenBank Accession LN492986 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106588-0022-485-E02C-8409
CGTGTTCACAAGTAAATAAACGGTTGCAAAATATCGACCAGAGATAAGGGGTGACCACTG
CTTTGAGACGCACTTGAACCTCATAAAGGACTTTGTTGGCAATCTTATAAGAATTTCTAT
CATGAGATCAGGAGGGATCTCGCAGTCGTGCCTATTGCACATATCTTTGATTCCTCTTCT
TCTTCTCTTCCTCTTCATACTCCTCTCTCGTTGCATGACTGCTGGACGAAAAACCAAACC
CAATAGATTTTGACAAAGCGCTACCCACAAACTTATATCAAAACCCTAAGTACTCTTGTT
CTTACTAATTAGGAAGAGGAAGTTCTTTCATGTTTCCCGGTCACGGTTGGTGGAGATATC
AAGGAGGCTAGCGTTGATTTTTTAAGCCCATTCGTTCAACAAAGCCCATTAAGAAACT
   
Sequence Name 31-K106589-0022-485-E02C-CC58
GenBank Accession LN500177 [GenBank]
Sequencing primer gene specific primer, CC58: 5'-AAGTTTCTTAATGGGCTTTGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K106589-0022-485-E02C-CC58
AACACGCTGCCTCCTTGAATCTCCACCAACCGTGACCGGGAAAATGAAAGAACTTCCTCT
TCCTAATTAGTAAGAACAAGAGTACTTAGGGTTTTGATATAAGTTTGTGGGTAGCGCTTT
GTCAAAATCTATTGGGTTTGGTTTTTCGTCCAGCAGTCATGCAACGAGAGAGGAGTATGA
AGAGGAAGAGAAGAAGAAGAGGAATCAAAGATATGTGCAATAGGCACGACTGCGAGATCC
CTCCTGATCTCATGATAGAAATTCTTATAAGATTGCCAACAAAGTCCTTTATGAGGTTCA
AGTGCGTCTCAAAGCAGTGGTCACCCCTTATCTCTGGTCGATATTTTTGCAACCGTTTAT
TTACTTGTGTAACACGACAACAACAACAACAACCACGTCTATACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11