Sequences from confirmation amplicon


Confirmation sequences for line 486B04 and hit At1g54220 are shown.
Line ID 486B04
Locus At1g54220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20246673
Primers used for PCR 18de - 8474, show primer details
   
Sequence Name 23-K109778-0022-486-B04C-18de
GenBank Accession LN496597 [GenBank]
Sequencing primer gene specific primer, 18de: 5'-TTAGAAAAACCCGAAAGAAACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109778-0022-486-B04C-18de
GAGACTCTTACGACAGCTTTTGATGTGTTTTTTTCCATTGCGTGGTTGGTTCCTTATAAA
GAGTAACATAGATTTAGGGTTCTCGATGTATCCCTCAAATGCTTTCAACCATTCAGCTCC
AATGGCACCTGCAGTAAGAAGAATAATATTAGATTTCTAGAATGATGATGAATGAAAGAA
TTATTGATG
   
Sequence Name 07-K109777-0022-486-B04C-8409
GenBank Accession LN489318 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109777-0022-486-B04C-8409
TTACAATTGAATATATCCTGGATCAATTCAACTTTGCTTCTTACATGCCTGTATACACTG
AGCTGTGACCATCGCGTAGTAGACGGTAATCCAGTTCATTCATCATCATTGATTCATTCA
TTCATCATCATTGTAGAAATCTAATATTCTTCTTCTTACTGCAGGTGCCATTGGAGCTGA
ATGGTTGAAAGCATTTAAGGGATACATCGAGAACCCTAAATCTATGTTACTCTAAAAGAG
AACCAACCACGCAATGGAAAAAAACACATCAAAAGCTGTCGTAAGAGTCTCTGTACCAAT
TAGATTTTCTTTGTTTTCCTCTGTTTCGTTCGGGTTTTTCTAAAAAGGCGGACGAAAGAA
CCTCTGTCCCATTTAAATTTTCTTTGT

Last Updated on 06.12.2018 16:11