Sequences from confirmation amplicon


Confirmation sequences for line 486D06 and hit At1g30730 are shown.
Line ID 486D06
Locus At1g30730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10902423
Primers used for PCR 29sr - 8474, show primer details
   
Sequence Name 66-K116549-0022-486-D06C-29sr
GenBank Accession LN511690 [GenBank]
Sequencing primer gene specific primer, 29sr: 5'-GTGCTACTAAACCCTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K116549-0022-486-D06C-29sr
GCGTCCGGTTTCGAGAACCGGCCTCGGTTTGATACTCAAGAAATTGGTTGAGTTGGAGAA
AGTTGAAATGAATTGGAATCCGTATGGAGGAAGAATGGGTGAGATCCCGAGTTCGAGGAC
ACCTTTCCCACATAGAGGAGGCAATTTGTTCAACATTGAATATATCATAGACTGGTCAGA
AGCTGGAGATAATGTGGAGAAGAAATATTTGGCGCTCGCAAATGAGTTTTATAGATTCAT
GACCCCGTACGTGTCTAGTAATCCGAGGGAGGCGTTTTTGAATTACCGTGATATTGACAT
TGGGTCAAGTGGTAATTCCACGTACGAGGAAGGTAAAATCTATGGGGCTAAATATTTCAA
AGACAATTTCGAGAGGTTAGTGGATATCAAAACCAAGTTCGATGAGATAAACTTTTGGAG
GAACGAACAAAGTATTCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 26-K116548-0022-486-D06C-8409
GenBank Accession LN498088 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116548-0022-486-D06C-8409
ATTTACATTGAATATTTCCCGGAATACTTTGTTCGTTCCTCCAAAAGTTTATCTCATCGA
ACTTGGTTTTGATATCCACTAACCTCTCGAAATTGTCTTTGAAATATTTAGCCCCATAGA
TTTTACCTTCCTCGTACGTGGAATTACCACTTGACCCAATGTCAATATCACGGTAATTCA
AAAACGCCTCCCTCGGATTACTAGACACGTACGGGGTCATGAATCTATAAAACTCATTTG
CGAGCGCCAAATATTTCTTCTCCACATTATCTCCAGCTTCTGACCAGTCTATGATATATT
CAATGTTGAACAAATTGCCTCCTCTATGTGGGAAAGGTGTCCTCGAACTCGGGATCTCAC
CCATTCTTCCTCCATACGGATTCCAATTCATTTCAACTTTCTCCAACTCAACCAATTTCT
TGAGTATCAAACCGAGGCCGGTTCTCGAAACCGGACGCTTCACGTAGTCCGATTTCCGTT
TCATGAACAACTTTTTTGTGACCGTAG

Last Updated on 06.12.2018 16:11