Sequences from confirmation amplicon


Confirmation sequences for line 486E03 and hit At5g07180 are shown.
Line ID 486E03
Locus At5g07180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2230267
Primers used for PCR SD06 - 8474, show primer details
   
Sequence Name 03-K023022-022-486-E03C-8409
GenBank Accession LN487305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K023022-022-486-E03C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCTGAGCTTGGCCAT
ATCATCAATCTTGATACATTGTATGTTATTTTCTACTGATTTACATCCATGTTCCGCCTA
GTTACCTACTGATATGTGTACCAACTACCAACTCATACAAACTTTGTTTCAGGGATCTGT
CTGGCAACAATTTCTCAGGCTCAATTCCATTAACACTTGGTGATCTTGAGCATCTTCTCA
TCTTGTATGTCTTCATCAAGCCCTAAAGTTGCACTGCTTTGCATTAACTCTCTCAGATTT
GTTGACTTGAGTTTTGTTTTAATTGTCAGAAACTTGAGCAGAAATCATCTGAATGGCACA
TTGCCTGCAGAATTCGGGAACCTCCGAAGCATTCAGATCATGTAAGGCTTGTCTTATGCT
TAGATTTCTTCTGTTATGATAAAATCTTCCTACTTACAAACTTTTCTCCATCCTTCTCAG
CGATG
   
Sequence Name 04-K023022-022-486-E03C-SD06
GenBank Accession LN487746 [GenBank]
Sequencing primer gene specific primer, SD06: 5'-AATTAAATGACACATCGCTGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K023022-022-486-E03C-SD06
ATCGGATGGAGAAAAGTTTGTAAGTAGGAAGATTTTATCATAACAGAAGAAATCTAAGCA
TAAGACAAGCCTTACATGATCTGAATGCTTCGGAGGTTCCCGAATTCTGCAGGCAATGTG
CCATTCAGATGATTTCTGCTCAAGTTTCTGACAATTAAAACAAAACTCAAGTCAACAAAT
CTGAGAGAGTTAATGCAAAGCAGTGCAACTTTAGGGCTTGATGAAGACATACAAGATGAG
AAGATGCTCAAGATCACCAAGTGTTAATGGAATTGAGCCTGAGAAATTGTTGCCAGACAG
ATCCCTGAAACAAAGTTTGTATGAGTTGGTAGTTGGTACACATATCAGTAGGTAACTAGG
CGGAACATGGATGTAAATCAGTAGAAAATAACATACAATGTATCAAGATTGATGATATGG
CCAAGCTCAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11