Sequences from confirmation amplicon


Confirmation sequences for line 487F04 and hit At3g59910 are shown.
Line ID 487F04
Locus At3g59910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22131793
Primers used for PCR 45jd - 8474, show primer details
   
Sequence Name 76-K113077-0022-487-F04C-45jd
GenBank Accession LN513429 [GenBank]
Sequencing primer gene specific primer, 45jd: 5'-AAACTCTACTCCTTGGAGAGACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113077-0022-487-F04C-45jd
TGCTGAAGCGGTTAAGACAGGTGGTGGAACACGAGGGAGGAGATAAGCAGCTACCTCGAC
TTGGCAACTTGAAGTGGCCGCTGTTAGAGCCAAGCAAAGCTCCATGTCACGGCAACCTCT
TTTCACAAACCATTGAACAACTTGCATGCAACCTCTCTCTGCTGCTCTCATTAAGGGACC
CAAGAAGGCAATGGCATTACCTTCTTCCACTAGGCACTTCATTGTTCCGATTTTACAATA
ATGCGAGGCAAAACCAAGAGCTAAGTCCACATCAACATCTAAGCTGTTCCTTTGGGCAAT
CTGCTCCAGGAAATACAATATTTGAGCACGAAAAGAGACCTTCTATAAGTCTAGAAGCAA
TCAAAAGAATGGAAGCTTACCTGCAATAAGACGCGGACAAGTTCTGTACTACCAAAACGT
GAAGCTTCAAGGAAACATTGGTTCACATTATCTGCGCCACCTTCAACAAGCATACCGAGC
AGTCCCTGTATAACAGTAGCTGACATCCCAGATGCCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTC
   
Sequence Name 49-K113075-0022-487-F04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K113075-0022-487-F04C-8409
ATACAGGGGGTGCTCGGTCTGGTTGTTGGGGGTGGCGCNNTGCCTGTGCCCCCCTGTTTC
CTT

Last Updated on 06.12.2018 16:11