Sequences from confirmation amplicon


Confirmation sequences for line 488H09 and hit At5g55260 are shown.
Line ID 488H09
Locus At5g55260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22417718
Primers used for PCR 17hj - 8474, show primer details
   
Sequence Name 46-K112551-0022-488-H09C-17hj
GenBank Accession LN505908 [GenBank]
Sequencing primer gene specific primer, 17hj: 5'-TATAACAGTAATTTGGGGCAGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K112551-0022-488-H09C-17hj
TTGTACCTTCCATCACTAGCTGATGAGCTCGACATATGTAATCAATGTTGTTTGAGTGGT
TAAAAGACGTAACAACACTGCCGCCGAAAAGGAATCCGGCACCACGGGGACTCAATCCCC
AACCATCGACAATATCTTCTGGATCAGACCATAAAAGATCACACATAGCACCATCATGTG
GTACTTCTTGCTTTCGATCAATAGCCCTGATCTGCCACAAGATATGTATGAAGTGATTAG
TCAGTTATATCCTTATAGCTAGAAACCTTATGATTGTATTATCACTGTCTCAACCTGGTC
TAGAGTCATAATAGCTGGAGAGAGACCTCCATGAACACAAAATATCTTGTTCTCGACAAG
AGCTGAAAGACTTAGACAAATAAAAGATAGGAAGTTTCCATTACATTAACCGAAATAATA
TCTACTCGGAACTCCAAAACAAAAGCAGAAAAAGGGTGTATACCTCAAGTAGTCAAAGAT
ATCTGTGCAGTATCTCCAAACATTTACAGAGCCATATTTACGCAGACACTCATCATAAAA
TCCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCC
   
Sequence Name 22-K112550-0022-488-H09C-8409
GenBank Accession LN496181 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K112550-0022-488-H09C-8409
TCATTGAATATATGGATTTTATGATGAGTGTCTGCGTAAATATGGCTCTGTAAATGTTTG
GAGATACTGCACAGATATCTTTGACTACTTGAGGTATACACCCTTTTTCTGCTTTTGTTT
TGGAGTTCCGAGTAGATATTATTTCGGTTAATGTAATGGAAACTTCCTATCTTTTATTTG
TCTAAGTCTTTCAGCTCTTGTCGAGAACAAGATATTTTGTGTTCATGGAGGTCTCTCTCC
AGCTATTATGACTCTAGACCAGGTTGAGACAGTGATAATACAATCATAAGGTTTCTAGCT
ATAAGGATATAACTGACTAATCACTTCATACATATCTTGTGGCAGATCAGGGCTATTGAT
CGAAAGCAAGAAGTACCACATGATGGTGCTATGTGTGATCTTTTATGGTCTGATCCAGAA
GATATTGTCGATGGTTGGGGATTGAGTCCCCGTGGTGCCGGATTCCTTTTCGGCGGCAGT
GTTGTTACGTCTTTTAACCACTCAAACAACATTGATTACATATGTCGAGCTCATCAGCTA
GTGATGGAAGGTTACAAATGGATGTTCAATAGCCAGATAGTCACTGTTTGGTCTGCCCCA
AATA

Last Updated on 06.12.2018 16:11