Sequences from confirmation amplicon


Confirmation sequences for line 488H12 and hit At1g71140 are shown.
Line ID 488H12
Locus At1g71140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26826124
Primers used for PCR HF43 - 8474, show primer details
   
Sequence Name 32-K101424-0022-488-H12C-8409
GenBank Accession LN500518 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K101424-0022-488-H12C-8409
TATGGTGGGTGCATAGTCTTTGGTGCAAGAAATGTATTTGGTTACCTATTCAGTTCTGTA
ACTGAAGTTGTAGACTATGTAAAATCAATGGCTCCACTGCTTTCTTTATCGGTCATATTC
GATGCCCTTCACGCAGCTCTCTCGGGTTAGATGATCTCCTTCTATAATGTTTTAAGAGAT
CTAATTCAATGAAGTTAAGAGTTATAAAATGTGTTTACTGAAACAGGTGTGGCTAGAGGA
TCAGGGAGGCAAGATATTGGGGCATACGTAAACCTAGCAGCATACTATCTCTTTGGAATA
CCAACTGCTATACTATTAGCTTTTGGGTTTAAAATGAGAGGAAGAGGACTCTGGATTGGA
ATCACAGTTGGGTCATGTGTACAAGCTGTTTTGCTCGGTCTTATCGTAATCCTCACTAAT
TGGAAAAAACAGGTTCGTTAAGTGAAAGTGATGTACTTTAATATGTTGATTTATGTTATG
GTCAAATAAATGGGCTAGTGG
   
Sequence Name 72-K101427-0022-488-H12C-HF43
GenBank Accession LN512694 [GenBank]
Sequencing primer gene specific primer, HF43: 5'-CCACTAGCCAATTTATTTGACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101427-0022-488-H12C-HF43
AGTCATCACTTTCCTTACGAACCTGTTTTTTCCATTAGTGAGGATTACGATAAGACCGAG
CAAAACAGCTTGTACACATGACCCAACTGTGATTCCAATCCAGAGTCCTCTTCCTCTCAT
TTTAAACCCAAAAGCTAATAGTATAGCAGTTGGTATTCCAAAGAGATAGTATGCTGCTAG
GTTTACGTATGCCCCAATATCTTGCCTCCCTGATCCTCTAGCCACACCTGTTTCAGTAAA
CACATTTTATAACTCTTAACTTCATTGAATTAGATCTCTTAAAACATTATAGAAGGAGAT
CATCTAACCCGAGAGAGCTGCGTGAAGGGCATCGAATATGACCGATAAAGAAAGCAGTGG
AGCCATTGATTTTACATAGTCTACAACTTCAGTTTCAGAACTGAATAGGTAACCAAATAC
ATTTCTTGCACCAAAGACTATGGCACCCACCATTATTGATTCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAA
ATGT

Last Updated on 06.12.2018 16:11