Sequences from confirmation amplicon


Confirmation sequences for line 489D08 and hit At5g47490 are shown.
Line ID 489D08
Locus At5g47490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19269613
Primers used for PCR 24et - 8474, show primer details
   
Sequence Name 23-K111225-0022-489-D08C-24et
GenBank Accession LN496609 [GenBank]
Sequencing primer gene specific primer, 24et: 5'-GAGTATCAACATCAACAGCAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K111225-0022-489-D08C-24et
ATGTCATCATTGATGCCACCTGCTTCAGTGGAACCCACACATGAGTCGGGTGGGAGTGGG
AGGAGAATGGCAGTACATACTAGAAGTGTATCAGAGCCAGATTTCGGTAGGACGCCGATA
CAGGTCTATACCAGAAAACCATAATCACCGCTAAGAATATTTGAAAATGGAATTTATAAA
AGGTTTCCCTTTTTTTAGGAAATGGCTGACTCCTCGAAAGAAAAGGCAGTTGATGGGGTG
ACAAAGTTGAAATCATCTGGGTCAGTGGCAGGTTCGCGGTTTAGTCGCTTTGGTTTTGGC
ATTTTTAAGGACACTGTAGGAAGGGTCTTAGCTCGGTCATCCAAAGAGGTACACTGCTAA
ATGCTTTGGTCGTGATGATATCTCTTGAACTTATTCTAATTTTTTCGGTTCATGCTTTGG
GTCAAGGCAAAATTGGGCGCAGAAAATCAGTTCTACTATGACGACAAACTAAAGAGATGG
GTGGAAAGAGGTGTGGAACCCCCAGCTGAGGAAGCTGCACTGCCTCCTCCTCCAACAATA
GGCGCATTCCAAAACAACTCATTGGGTTATGAAAACAAATCTGATATGATTCCATCTAAT
GGGAACTGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCACGGTTATT
   
Sequence Name 07-K111224-0022-489-D08C-8409
GenBank Accession LN489331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K111224-0022-489-D08C-8409
TCGTTCATTGATGGATCATATCAGATTTGTTTTCATAACCCAATGAGTTGTTTTGGAATG
CGCCTATTGTTGGAGGAGGAGGCAGTGCAGCTTCCTCAGCTGGGGGTTCCACACCTCTTT
CCACCCATCTCTTTAGTTTGTCGTCATAGTAGAACTGATTTTCTGCGCCCAATTTTGCCT
TGACCCAAAGCATGAACCGAAAAAATTAGAATAAGTTCAAGAGATATCATCACGACCAAA
GCATTTAGCAGTGTACCTCTTTGGATGACCGAGCTAAGACCCTTCCTACAGTGTCCTTAA
AAATGCCAAAACCAAAGCGACTAAACCGCGAACCTGCCACTGACCCAGATGATTTCAACT
TTGTCACCCCATCAACTGCCTTTTCTTTCGAGGAGTCAGCCATTTCCTAAAAAAAGGGAA
ACCTTTTATAAATTCCATTTTCAAATATTCTTAGCGGTGATTATGGTTTTCTGGTATAGA
CCTGTATCGGCGTCCTACCGAAATCTGGCTCTGATACACTTCTAGTATGTACTGCCATTC
TCCTCCCACTCCCACCCGACTCATGTGTGGGTTCCACTGAAGCAGGTGGCATCAATGATG
ACATTGTATTAACCGATTGACTATATGCTAACTTAGTAGCTTCCTGCTGTTGATGTTGAT
ACT

Last Updated on 06.12.2018 16:11