Sequences from confirmation amplicon


Confirmation sequences for line 490C09 and hit At5g28520 are shown.
Line ID 490C09
Locus At5g28520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10494694
Primers used for PCR HF41 - 8474, show primer details
   
Sequence Name 34-K101425-0022-490-C09C-8409
GenBank Accession LN501353 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101425-0022-490-C09C-8409
CATTTACAATTGAACCGAAGCCTGGTTATAACTCCGCGTTCATTTCCACTTGTTTTGTCC
CAAAAACCTTCCACCGTAGTGATGTATTCACTCGGATAATCTAGCTTGAACTGCAAAACA
TGGAGAATAAGTAAAGTGGTTGATCATAAAGAACAATTGTAACAAGGAAGCTCTGAATCT
TTAGCCGATACATATGTTACTCTAGGTCAAGGAAAAGCATTTGGTAATACAAATTTAAAG
TAAATACGTGAATGAATATATAATCACCTCATTGAATCCTATTCCTGATGGTTCCCCATG
AACTTTCAGTTCAGTCACCTGGACGTCCTTATCGTACACAAACTTGAGGGCGATACCCTC
ACCGTCTTCTAGATG
   
Sequence Name 38-K101428-0022-490-C09C-HF41
GenBank Accession LN502917 [GenBank]
Sequencing primer gene specific primer, HF41: 5'-CATCTAGAAGACGGTGAGGGTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101428-0022-490-C09C-HF41
ATAGGAGTCCGGTGACTGAACTGAAAGTTCATGGGGAACCATCAGGAATAGGATTCAATG
AGGTGATTATATATTCATTCACGTATTTACTTTAAATTTGTATTACCAAATGCTTTTCCT
TGACCTAGAGTAACATATGTATCGGCTAAAGATTCAGAGCTTCCTTGTTACAATTGTTCT
TTATGATCAACCACTTTACTTATTCTCCATGTTTTGCAGTTCAAGCTAGATTATCCGAGT
GAATACATCACTACNGTGGAAGGTTTTTGGGACAAAACAAGTGGAAATGAACGCGGAGTT
ATAACCAGGCTTCGGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAGAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATCTCCGCAGCGTTGTTATATGTCGNGCC

Last Updated on 06.12.2018 16:11