Sequences from confirmation amplicon


Confirmation sequences for line 491E10 and hit At4g17880 are shown.
Line ID 491E10
Locus At4g17880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9935203
Primers used for PCR MH28 - 8474, show primer details
   
Sequence Name 19-K020243-022-491-E10C-8409
GenBank Accession LN494569 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K020243-022-491-E10C-8409
CATTTACGCCGTGACCAACATATATTATCGCCGGAGAAGACAACAACAACAACAACACAG
TGTTGTTAGGTTGGGGAGATGGTTATTACAAAGGAGAAGAAGAGAAGTCTAGAAAGAAGA
AATCAAATCCAGCTAGTGCAGCTGAACAAGAGCATCGTAAGAGAGTGATTAGAGAGCTCA
ACTCTTTAATCTCCGGTGGTGTAGGAGGAGGAGATGAAGCTGGAGATGAAGAAGTTACAG
ATACTGAATGGTTCTTCTTAGTTTCAATGACACAGAGCTTTGTCAAGGGTACTGGTTTAC
CTGGTCAAGCTTTCTCAAATTCAGACACGATTTGGTTATCTGGTTCTAATGCTTTAGCTG
GATCA
   
Sequence Name 23-K020243-022-491-E10C-MH28
GenBank Accession LN496373 [GenBank]
Sequencing primer gene specific primer, MH28: 5'-TCTCTCACAACTTGATCCAGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K020243-022-491-E10C-MH28
ATTAGAACCAGATAACCAAATCGTGTCTGAATTTGAGAAAGCTTGACCAGGTAAACCAGT
ACCCTTGACAAAGCTCTGTGTCATTGAAACTAAGAAGAACCATTCAGTATCTGTAACTTC
TTCATCTCCAGCTTCATCTCCTCCTCCTACACCACCGGAGATTAAAGAGTTGAGCTCTCT
AATCACTCTCTTACGATGCTCTTGTTCAGCTGCACTAGCTGGATTTGATTTCTTCTTTCT
AGACTTCTCTTCTTCTCCTTTGTAATAACCATCTCCCCAACCTAACAACACTGTGTTGTT
GTTGTTGTTGTCTTCTCCGGCGATAATATATGTTGGTCACGGCGTAAATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11