Sequences from confirmation amplicon


Confirmation sequences for line 492C04 and hit At3g06180 are shown.
Line ID 492C04
Locus At3g06180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1873071
Primers used for PCR KG15 - 8474, show primer details
   
Sequence Name 64-K038030-022-492-C04C-KG15
GenBank Accession LN511147 [GenBank]
Sequencing primer gene specific primer, KG15: 5'-GAAGATAACGGCGTAAACTGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K038030-022-492-C04C-KG15
ACAAATGACTTCAGAACACAATGTCAACCAAAAATACTTTGATTTGATTATGGATTGAAA
GAGAGATGTGAGTCAAACAAAGAAAGAGAACAAGTCTGATAAGAACACATATGACAAAAC
AAATCACAAAATCTACTGAGAGTAACCAACCAACGACCTTAGTCAGTAGATGAGTAGATA
ATATTACAAGTTACACACAAAGCAATAAAAAGAAGGCATTCATCTACTCAAGGTTGTTGA
CAACACCATGTATTGTGCTTTATAAAAGCATGTATTGTTATCGAGTAAATCTCCAATTTT
TAGATATTGTTGGTGCTAGGCTGTATAATAATACTCAAGGTTGAAGTCAAGGGAGCCAGA
AATGACCATTGAAAACAAGGTCCAAGTTCCATGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 08-K038030-022-492-C04C-8409
GenBank Accession LN489652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K038030-022-492-C04C-8409
GCCATTTACAATTGAATATATCATGGAACTTGGACCTTGTTTTCAATGGTCATTTCTGGC
TCCCTTGACTTCAACCTTGAGTATTATTATACAGCCTAGCACCAACAATATCTAAAAATT
GGAGATTTACTCGATAACAATACATGCTTTTATAAAGCACAATACATGGTGTTGTCAACA
ACCTTGAGTAGATGAATGCCTTCTTTTTATTGCTTTGTGTGTAACTTGTAATATTATCTA
CTCATCTACTGACTAAGGTCGTTGGTTGGTTACTCTCAGTAGATTTTGTGATTTGTTTTG
TCATATGTGTTCTTATCAGACTTGTTCTCTTTCTTTGTTTGACTCACATCTCTCTTTCAA
TCCATAATCAAATCAAAGTATTTTTGGTTGACATTGTGTTCTGAAGTCATTTTGTTCTTC
TCTTTGCATACTGATTCAGTTTACGCCGTTTATCTT

Last Updated on 06.12.2018 16:11