Sequences from confirmation amplicon


Confirmation sequences for line 492D11 and hit At1g33811 are shown.
Line ID 492D11
Locus At1g33811 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12268800
Primers used for PCR HF37 - 8474, show primer details
   
Sequence Name 35-K101425-0022-492-D11C-8409
GenBank Accession LN501766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101425-0022-492-D11C-8409
GGATGAGCCATTTACAATTGAATTTCTTCTATGAACCAGCAGGTAGAGCTATACACGACC
GCGGTTCAACAGATGCTTCGATACTTCAGAGGAGACACAAATGAGCTCCAGAGATACTTA
AGCCGTTGTATCTTTTACTCGGGAATGGGAAGTAACGATTATCTCAACAATTACTTCATG
CCTGACTTTTACTCAACCAGTACCAATTACAATGACAAAACCTTTGCTGAGTCTCTGATA
AAGAACTACACACAACAGCTCACGGTAAGGGTTGATCAGTAAGACAATAATGCATATATC
TATCTCAATAGATTTTCTATATCTTGATTCTTTTTTTTTTCTTTTTTTCGTAGCGATTGT
ACCAATTTGGAGCTCGGAAAGTGATTGTGACTGGAGTGGGACAGATCGGTTGCATACCTT
ACCAGCTCGCCCGCTACAATAATCGTAACAATAGTACTGGCAGATGCAACGAAAAGATTA
ACAACGCATTTGTGGTGTTTAACACTCAAGTCAAGAAACTAGTGGACCGTTTAAACAAAG
GTCAGTTGAAGGGAGCAAAGTTTGTTTACCTTGATTCCTACAAGAGTACCTATGAT
   
Sequence Name 39-K101428-0022-492-D11C-HF37
GenBank Accession LN503295 [GenBank]
Sequencing primer gene specific primer, HF37: 5'-TGGATGTTGATTGGGTAAGTGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K101428-0022-492-D11C-HF37
CAGTCTCCGTCGGATGAACGCGTCCAAAAGAGATACTTGGTTCGGTCAGGGCACGGGGTC
TGCAGCGGCAAACATGTTATCTGACCATTGTTCCTCCCCACCCCACAACAACCTTTGTCC
ACAACCTCAAATCCTGTAGGATCAATGTAAATAACTAGAAGACATTTTAAAAACAATTAG
ACTAATATATTTTCAATGTATGTAACTCACCGTAAGCGGCTCCATTGACGGCGAGATCAT
AGGTACTCTTGTAGGAATCAAGGTAAACAAACTTTGCTCCCTTCAACTGACCTTTGTTTA
AACGGTCCACTAGTTTCTTGACTTGAGTGTTAAACACCACAATTGCGTTGTTAATCTTTT
CGTTGCATCTGCCAGTACTATTGTTACGATTATTGTAGCGGGCGAGCTGGTAAGGTATGC
AACCGATCTGTCCCACTCCAGTCACAATCACTTTCCGAGCTCCAAATTGGTACAATCGCT
ACGAAAAAAAGAAAAAAAAAAGAATCAAGATNTAGAAAATCTATGGAGATAGATATATGC
ATTATTGTCTTACTGATCAACCCTTACCGTGAGCTGTTGTGTGTAGTTCTTTATCAGAGA
CTCAGCAAAGGTTTTGTCATTGTAATTGGTACTGGTTGAGTAAAAGTCAGGCATGAAGTA
ATTGTTGAGATAATCCTTACTTCCCATTCCCGAGTAAAAA

Last Updated on 06.12.2018 16:11