Sequences from confirmation amplicon


Confirmation sequences for line 492F04 and hit At1g79650 are shown.
Line ID 492F04
Locus At1g79650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29973101
Primers used for PCR AH06 - 8474, show primer details
   
Sequence Name 68-K105568-0022-492-F04C-AH06
GenBank Accession LN512018 [GenBank]
Sequencing primer gene specific primer, AH06: 5'-CTAAGGCTCTATCAAATCCCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K105568-0022-492-F04C-AH06
AGTTAAAAGGGTTGCAAAGAAAAAAGTGTGGAAACAGAGTCTTACTATTAATGGTGTTTT
ACCCGTTGAATCGCTTCTTGTTCTGCTGGGGTAACGTTAATGGCATGTGGCATTTCTTGC
TCGGGTTGATCGAACATGTCCCCCTCCCTGATGAGTTATGTTTTTAAACTTTGTGAATCA
AATTATTTAGTTTAATGAAATATCAATAAGAAGCATATAATTACATCTGACAGAACCTTA
GATTACAATAAACAGATTGGAGGACTCACCCGTCAGAACCCTCATAGGGCTCATTTACTA
ACTGAAGAAACTCTGCCTGGTTTTCTTGAATTAGCCTCAGAAGTTGAGGGTTTTGCTTTC
CAAGTTCCTGAAGCATGGGCTGGTAACAAAAGCAGTTGTTCCAACATAAGAGGTTCGGAA
TCAGTGAGAAGTCTTACAAACTACTGGACCAGGATAACGAAAACTAGACGAGTCTTACCT
GAAGAATTTGAGGGTTGGAATGGACCATGGTGCGTAATTGTTGGAACTAATGTAATAGCA
AATACAAGATCATGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTTATT
   
Sequence Name 84-K105569w-0022-492-F04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K105569w-0022-492-F04C-8409
TTTACATTGAATACATGATCTTGTATTTGCTATTACATTAGTTCCAACAATTACGCACCA
TGGTCCATTCCAACCCTCAAATTCTTCAGGTAAGACTCGTCTAGTTTTCGTTATCCTGGT
CCAGTAGTTTGTAAGACTTCTCACTGATTCCGAACCTCTTATGTTGGAACAACTGCTTTT
GTTACCAGCCCATGCTTCAGGAACTTGGAAAGCAAAACCCTCAACTTCTGAGGCTAATTC
AAGAAAACCAGGCAGAGTTTCTTCAGTTAGTAAATGAGCCCTATGAGGGTTCTGACGGGT
GAGTCCTCCAATCTGTTTATTGTAATCTAAGGTTCTGTCAGATGTAATTATATGCTTCTT
ATTGATATTTCATTAAACTAAATAATTTGATTCACAAAGTTTAAAAACATAACTCATCAG
GGAGGGGGACATGTTCGATCAACCCGAGCAAGAAATGCCACATGCCATTAACGTTACCCC
AGCAGAACAAGAAGCGATTCAACGGGTAAAACACCATTAATAGTAACACTCTGTTTCCAC
ACTTTTTTCTTTGCAACCCTTATTAACTATATCCCTGGCCTCTGGCCTATCTAACAGCTC
GAGGCAATGGGATTTGATAGAGCCTTAGATNGGCNNGAGGCGAGG

Last Updated on 06.12.2018 16:11