Sequences from confirmation amplicon


Confirmation sequences for line 492F05 and hit At1g65080 are shown.
Line ID 492F05
Locus At1g65080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24176595
Primers used for PCR 17qw - 8474, show primer details
   
Sequence Name 46-K115837-0022-492-F05C-17qw
GenBank Accession LN505964 [GenBank]
Sequencing primer gene specific primer, 17qw: 5'-CCATAGAGCGATGTCTGTCAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115837-0022-492-F05C-17qw
CTAAACCTAGTAGAAAGGTCATGACTTTGGTATCAATAAAGAAACTGCAAATTATAAAAA
CTACTTATAAAGTAGAGAAATAATACTAACACTTCGGTAGCAGCTCTGAAATGGTCTTCA
ATTTCTTGAGTTGAAGTATGAGTAAAGGCAATAAAGCTAATCTAACAGCAACAGTAGAAG
ATGCAATAATCATCCACCAAGGCAATCCTGTGAATTCATGAAAGCCTTCGAGGAAATCAA
CGACGGCGAGAACAGGTAAAGAAGAATCATCGGTAGGGATGATTTCTTGAGACCCAATTG
GGAATTCTGGTCCAGAAGAGAGTAATCGGTGGAGAGGAGAGAAACACGTCGGAGATGGTG
ACGGCGAGGGAGAGAAGAGAGGAGGAGAATGGGAGATGAAGGAGGAGGGTTTGAGGTGGT
GG
   
Sequence Name 14-K115833-0022-492-F05C-8409
GenBank Accession LN492650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115833-0022-492-F05C-8409
GTTTAGGCAAAGAAAAAAACTGGAATGGCAGTGTGGAGGAGTTGTTTACGCGCCTCCTCC
TCTCGTCACCGTTTATCTTCTCTCTTCTTCTCCCACCACCTCAAACCCTCCTCCTTCATC
TCCCATTCTCCTCCTCTCTTCTCTCCCTCGCCGTCACCATCTCCGACGTGTTTCTCTCCT
CTCCACCGATTACTCTCTTCTGGACCAGAATTCCCAATTGGGTCTCAAGAAATCATCCCT
ACCGATGATTCTTCTTTACCTGTTCTCGCCGTCGTTGATTTCCTCGAAGGCTTTCATGAA
TTCACAGGATTGCCTTGGTGGATGATTATTGCATCTTCTACTGTTGCTGTTAGATTAGCT
TTATTGCCTTTACTCATACTTCAACTCAAGAAATTGAAGACCATTTCAGAGCTGCTACCG
AAGTGTTAGTATTATTTCTCTACTTTATAAGTAGTTTTTATAATTTGCAGTTTCTTTATT
GATACCAAAGTCATGACCTTTCTACTAGGTTTAGTTGTTTCAATGTTAGTCATGGAACTC
GTTTACATGTGAAACAATAATTGACAGACATCCNTCTATGG

Last Updated on 06.12.2018 16:11