Sequences from confirmation amplicon


Confirmation sequences for line 493G08 and hit At1g01570 are shown.
Line ID 493G08
Locus At1g01570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:207167
Primers used for PCR 09md - 8474, show primer details
   
Sequence Name 60-K114263-0022-493-G08C-09md
GenBank Accession LN510322 [GenBank]
Sequencing primer gene specific primer, 09md: 5'-ATTTACAAGCTCGAAATTGCTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K114263-0022-493-G08C-09md
CAAATACGATTGGTACAAGAAACGCTATAAAAGATAAATAATTCTATTTGACAAGGAATT
CAGCAAATTCATCATCTTTACAAGCTCCTACATCGATTACCATAGTCCCATTCTTCGTCG
TGGGCAATACTCTACAACAATCTCTTCTCGGAGCCTGAAATTATCACCACAAAATTATGA
ATCAATAAAACTTATAGAGAAACAAAACTTAAAAATGATAACATTGTTTACTTACCCTAT
GTTTATTCCATCTATCCGGGTCGGGTTTCTTATAAACAATGACCCGTTCAAATTCCGAAG
GATCCGACATATTCAATTGACCATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 17-K114262-0022-493-G08C-8409
GenBank Accession LN493943 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114262-0022-493-G08C-8409
ATGATGGTCAATTGAATATGTCGGATCCTTCGGAATTTGAACGGGTCATTGTTTATAAGA
AACCCGACCCGGATAGATGGAATAAACATAGGGTAAGTAAACAATGTTATCATTTTTAAG
TTTTGTTTCTCTATAAGTTTTATTGATTCATAATTTTGTGGTGATAATTTCAGGCTCCGA
GAAGAGATTGTTGTAGAGTATTGCCCACGACGAAGAATGGGACTATGGTAATCGATGTAG
GAGCTTGTAAAGATGATGAATTTGCTGAATTCCTTGTCAAATAGAATTATTTATCTTTTA
TAGCGTTTTCTTGTTACCAATCGTATTTTTGAAATTTCGAGCTTATGTTTTAACAGCAAT
TTCGACNTTGTAAA

Last Updated on 06.12.2018 16:11