Sequences from confirmation amplicon


Confirmation sequences for line 495A10 and hit At3g27170 are shown.
Line ID 495A10
Locus At3g27170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10026264
Primers used for PCR MD15 - 8474, show primer details
   
Sequence Name 03-K024846-022-495-A10C-8409
GenBank Accession LN487323 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024846-022-495-A10C-8409
CATTTCCCGGACATGAAGCCATATACAATTGAATATACAATTGAATGTGGATGTTGTAAC
CCTGCAAGGTGTTGAAAAGGTTTCAAATATAGTTGATGTGCTAAAGAACACGACGCATAA
TGCATTCCCTGTTTTAGATGAAGCAGAAGTACCTCAAGTGGGTCTAGCAACTGGGGCTAC
AGAACTCCACGGGTTGATCTTGAGAGCGCACCTCGTTAAAGTTCTGAAAAAGAGATGGTT
CTTGACAGAGAAAAGAAGAACAGAGGAGTGGGAGGTCAGAGAAAAGTTTCCATGGGATGA
ATTGGCTGAAAGAGAAGACAACTTTGACGACGTGGCCATCACAAGCGCTGAAATGGAAAT
GTATGTCGATCTTCATCCTCTCACCAACACAACACCTTACACAGTCATGGA
   
Sequence Name 04-K024846-022-495-A10C-MD15
GenBank Accession LN487762 [GenBank]
Sequencing primer gene specific primer, MD15: 5'-TGACATGTTCTCCATGACTGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024846-022-495-A10C-MD15
TGTTGTGTTGGTGAGAGGATGAAGATCGACATACATTTCCATTTCAGCGCTTGTGATGGC
CACGTCGTCAAAGTTGTCTTCTCTTTCAGCCAATTCATCCCATGGAAACTTTTCTCTGAC
CTCCCACTCCTCTGTTCTTCTTTTCTCTGTCAAGAACCATCTCTTTTTCAGAACTTTAAC
GAGGTGCGCTCTCAAGATCAACCCGTGGAGTTCTGTAGCCCCAGTTGCTAGACCCACTTG
AGGTACTTCTGCTTCATCTAAAACAGGGAATGCATTATGCGTCGTGTTCTTTAGCACATC
AACTATATTTGAAACCTTTTCAACACCTTGCAGGGTTACAACATCCACATTCAATTGTAT
ATTCAATTGTATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTAC
Primers used for PCR MD14 - 8474, show primer details
   
Sequence Name 31-K024845-022-495-A10C-8409
GenBank Accession LN500001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K024845-022-495-A10C-8409
GATTTCCCGGACATGAAGCCATTTCCCTGTTCTGTAGCCAGTTGAATATATACTCATTGC
AGCATCACCAAGCTCACCAACGGTGAGGTTCCTCATCCACGGCTCTGGATTTGCTTCTAA
GAAAGGTAAGCCCTTTAGATGCAAGATGATGTCATATATACTCGGGTTAAAGCTGTCTCC
CACAGTTTTGGCTATCAGAAGCACGATCATCGTTATAGGAAGCAAAAGAAGGTTGTTGGT
GAGTTCAAGGAATATAACACAGAGTGACACAGTCATTCTCATCGATCCAGCCATGAGTGC
AGCTGCACCAAGGACAGCATAAAGCCCTTGGTCAATACTTGTGTATGATCCCATTGCAGC
GCCAAGCATTCGGCCATATGCAGCACCCATGAGGATGATGGGGAGGAAGAGACCAGACGG
TGTTGCAATACCAAATGTGAAAAGCCCCAAGATGCAGTATAGCACAAAGAATATCCAAAG
GGAACCCATACCAAACTCATTGGGAGTGTTGGAAGAGAAAAGGTTTCTGACAGCATCATC
GTTGGTGGTGAGAAGCAGAGTAGCTAGATCATTGTAGTAACCTTTAGGGCAATGGAACTG
TTTGAAGTTACCCGATCTTCCATTCGTCGGGCATATCTCATCTATCGAGGGGTCACAAGG
CTTGCATTTCGCTAAGAAAGGAAGGCCATAAAGGCAAACAGATGTAAAGAGTGATACTGT
AAGACTGAGAAGCACCTTATGGATCTTACCCTTCCTGCAAATGACAAACAAAGTAAGTAA
CTA
   
Sequence Name 32-K024845-022-495-A10C-MD14
GenBank Accession LN500425 [GenBank]
Sequencing primer gene specific primer, MD14: 5'-CCTGTCATGTTGATTGGTGTAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K024845-022-495-A10C-MD14
TGGAATTCGTTGGGAGCCTGTACAATCATCTTCTGCATAAAGTTCTCAGGCTTTACAATC
TCATCAATGAGTAAGTGTTAGCTATTCAATGAGTCAAAACCCGTTAAGCTAGTTACTTAC
TTTGTTTGGTCATTTTGCAGGAAGGGTAAGATCCATAAGGTGCTTCTCAGTCTTACAGTA
TCACTCTTTACATCTGTTTGCCTTTATGGCCTTCCTTTCTTAGCGAAATGCAAGCCTTGT
GACCCCTCGATAGATGAGATATGCCCGACGAATGGAAGATCGGGTAACTTCAAACAGTTC
CATTGCCCTAAAGGTTACTACAATGATCTAGCTACTCTGCTTCTCACCACCAACGATGAT
GCTGTCAGAAACCTTTTCTCTTCCAACACTCCCAATGAGTTTGGTATGGGTTCCCTTTGG
ATATTCTTTGTGCTATACTGCATCTTGGGGCTTTTCACATTTGGTATTGCAACACCGTCT
GGTCTCTTCCTCCCCATCATCCTCATGGGTGCTGCATATGGCCGAATGCTTGGCGCTGCA
ATGGGATCATACACAAGTATTGACCAAGGGCTTTATGCTGTCCTTGGTGCAGCTGCACTC
ATGGCTGGATCGATGAGAATGACTGTGTCACTCTGTGTTATATTCCTTGAACTCACCAAC
AACCTTCTTTTGCTTCCTATAACGATGATCGTGCTTCTGATAGCCAAAACTGTGGGAGAC
AGCNTTTACCCGAGTATATATGACATCATCTTGCATCTAAAGGGCTTACCTTT

Last Updated on 06.12.2018 16:11