Sequences from confirmation amplicon


Confirmation sequences for line 495D03 and hit At4g34790 are shown.
Line ID 495D03
Locus At4g34790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16594549
Primers used for PCR GA73 - 8474, show primer details
   
Sequence Name 43-K101501-0022-495-D03C-8409
GenBank Accession LN504781 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101501-0022-495-D03C-8409
GCCATTTACAATTGAATATATCCTGAGGTCGATGCTTCCAAATGCAAAGCAAATCTTCAA
ATCACAATCTATGAGGAACAAGAACGGATCATCCTCACCATCAACAACAACAACAACTTC
AGGGCTTGTTCCGAAAGGTCACGTAGCGGTTTACGTTGGAGAACAAATGGAGAAGAAGAG
GTTTGTGGTTCCAATATCATACTTGAACCATCCTTTGTTCAGAGAATTTCTTAATCGCGC
TGAGGAAGAATGCGGATTTCATCACTCTATGGGTGGCTTGACGATACCTTGTCGAGAAGA
GTCGTTTCTTTATCTCATTACTTCTCATCAGCTGCATTGAGGAACGCATTAAGTGATGTA
ATAGTCTTGAGGGATTTAATTAAGCTGTTTTTCTTTCCTCTTTTAAGAGGAGAATTATAG
GGAAGTTACTTATGTGTGTATATGGTGACACTTGTTCAGAGCTTAAGTTGTATTTTGAAA
TAAGTTTTTTTTTTTTTTTTTTAAAAAAAATTTGAAAAGCTTGTTGAGCTAAAATTTATT
TTCCAATCCTTAAAAGCTTTTAACTTTTAGCCTTTTACAGGAAAAATGGGGGAAAAAAAA
CCAAACTTTAAATGGGGGAAAAA
   
Sequence Name 43-K101505-0022-495-D03C-GA73
GenBank Accession LN504782 [GenBank]
Sequencing primer gene specific primer, GA73: 5'-TTTTTACCGCATTTAAAGTTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101505-0022-495-D03C-GA73
GTTAAAAGCTTTTAATGATTTGAATATAAATTTNTAGCTCAACTAGCTTTTCAAATCTTA
TTTAAAAAAAAAAAAAAAAAACTTTTTTCAAAATACAACTTAAGCTCTGAACAAGGGTCC
CCATATACCCCCATAAGTAACTTCCCTATAATTCTCCTCTAAAAAGAGGAAAGAAAAACG
GCTTATTTAAATCCCTCAAGACTATAACTTCATTTAAGGGGTTCCTCAATGCACCTAATG
AGAAGTAATGAGATAAAGAAACGACTCTTCTCGACAAGGTTTCGTCAAGCCCCCCATAGG
GGGATGAAATCCGCATTCTTCCTCAGCGCGATAAAAAAATTCTCTGAACAAAGGAGGGTT
CAAGTATGATTTTGGAACCACAAACCTCTTCTTCTCCATTTGTTCCCCAACGTAAACCGC
TTCGTGACCTTTCGGAAAAAGCCCTGAATTTTTTTTTTTTTTTGATGGGGAGGAAGATCC
CTTTTTGTTCCCCAAAGATGGGGATTTGAAAATTTGCTTTGCATTTGGA

Last Updated on 06.12.2018 16:11