Sequences from confirmation amplicon


Confirmation sequences for line 496F06 and hit At5g67380 are shown.
Line ID 496F06
Locus At5g67380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26883359
Primers used for PCR 13du - 8474, show primer details
   
Sequence Name 78-K110680-0022-496-F06C-13du
GenBank Accession LN513704 [GenBank]
Sequencing primer gene specific primer, 13du: 5'-ACTATATTTTCCACGCCCTACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K110680-0022-496-F06C-13du
GCAATAACAAGATCAAAATGTTAAACTAAAAAATCATTGCTTTACAATAGAAGAACTAAA
AGGCAAAATAAGCACAACTAAAAAATCAAATTCATCAATAGAGATAAAAGTAAAGTTTTT
TACATTAGCAGCATCAATTTGATTAGATTAGCTTCATCAAGAAGCAAATCCAGAGCAAGC
TGAATTCAAATTCAATACAAACACTACAAAAATCGATTAAATTACTTTAGCAACTACCAA
TTCAGCTAATTCTAGGGTTTAACAATCTTACCCCCATTGAACAATGAGTGACTCGTAATC
CCAATAATCCTTGGGTCGAATCACATTGACTTCGGTGTACACACGAGCTTTCGACATCGC
CCGCGTCTGGTTAGAATCAGAGATCTCTACGAGAAAATCTGGATCGATCGTAGTACAACC
GGAAACTTCCGAGACAGCTCTCGCTGTGGGAGTGACGATAGATGAGCGTAGGATAGGAGA
GTGCGCCGTTGGCGCACGTAAGGCTAAGACGGCGCACAGTAACAAGAGACGTCGAAGAGG
AGAATCGAAGAAGAGGAAGAAAAAGTTAATCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 70-K110679-0022-496-F06C-8409
GenBank Accession LN512412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K110679-0022-496-F06C-8409
CGGACTGAGCCTTTACATTGAATATATCCTGATTAACTTTTTCTTCCTCTTCTTCGATTC
TCCTCTTCGACGTCTCTTGTTACTGTGCGCCGTCTTAGCCTTACGTGCGCCAACGGCGCA
CTCTCCTATCCTACGCTCATCTATCGTCACTCCCACAGCGAGAGCTGTCTCGGAAGTTTC
CGGTTGTACTACGATCGATCCAGATTTTCTCGTAGAGATCTCTGATTCTAACCAGACGCG
GGCGATGTCGAAAGCTCGTGTGTACACCGAAGTCAATGTGATTCGACCCAAGGATTATTG
GGATTACGAGTCACTCATTGTTCAATGGGGGTAAGATTGTTAAACCCTAGAATTAGCTGA
ATTGGTAGTTGCTAAAGTAATTTAATCGATTTTTGTAGTGTTTGTATTGAATTTGAATTC
AGCTTGCTCTGGATTTGCTTCTTGATGAAGCTAATCTAATCAAATTGATGCTGCTAATGT
AAAAAACTTTACTTTTATCTCTATTGATGAATTTGATTTTTTAGTTGTGCTTATTTTGCC
TTTTAGTTCTTCTATTGTAAAGCAATGATTTTTTAGTTTAACATTTTGATCTTGTTATTT
GCTCTCTCAGGGAGCAAGATGATTATGAGGTAGTAAGGAAGGTAGGGCGTGAAAAATATA
G

Last Updated on 06.12.2018 16:11