Sequences from confirmation amplicon


Confirmation sequences for line 497A11 and hit At3g63150 are shown.
Line ID 497A11
Locus At3g63150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23332350
Primers used for PCR EQ81 - 8474, show primer details
   
Sequence Name 52-K103224-0022-497-A11C-EQ81
GenBank Accession LN507781 [GenBank]
Sequencing primer gene specific primer, EQ81: 5'-ATCACTCTAGGCATGGTTCAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103224-0022-497-A11C-EQ81
GGTTCCTAGTATCTATACGGAACATTTTCTCTCTTAGCCAAAAGATAATCTTTAAACTGT
AGCAGAAGATATTTCGGTCAGAGAACAAAAGCAAGAAGATACCTGTATAAGGGTCAATGC
AGAACACTCAATGCAGGTTTCTATCTCTCGGTACTCCTTCATGATTGGTGACATAATATC
CTCTAGTCTGGCGGGAGATCGTTCATCACGTAAATCAAGCTTGCAGCCCACCACAATCAC
AGGTGCTTTGATCTAGATACATTACATACATATATNATTATAACTTGAAAACATACTTGT
AGGAGAATGCGAAGATACGATATTTCAGCAATAAATAAATAAGAAACCTCTAAACGCCGG
AGCTCAGGGAGCCAATAAGAACTCAAGCGATCAAGAGTTGAAGGCTGATCACAAGCATAA
GTGAGAAGGACCACATCAGCCTTCCTGAATTCTTCAATGAGCTTAATCCGATTGTCAATA
CTAATAGCAGATAAACATGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 20-K103225-0022-497-A11C-8409
GenBank Accession LN495171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103225-0022-497-A11C-8409
ATTGACATCGGATAAGCTCATTGAAGAATTCAGGAAGGCTGATGTGGTCCTTCTCACTTA
TGCTTGTGATCAGCCTTCAACTCTTGATCGCTTGAGTTCTTATTGGCTCCCTGAGCTCCG
GCGTTTAGAGGTTTCTTATTTATTTATTGCTGAAATATCGTATCTTCGCATTCTCCTACA
AGTATGTTTTCAAGTTATAATTATATATGTATGTAATGTATCTAGATCAAAGCACCTGTG
ATTGTGGTGGGCTGCAAGCTTGATTTACGTGATGAACGATCTCCCGCCAGACTAGAGGAT
ATTATGTCACCAATCATGAAGGAGTACCGAGAGATAGAAACCTGCATTGAGTGTTCTGCA
TTGACCCTTATACAGGTATCTTCTTGCTTTTGTTCTCTGACCGAAATATCTTCTGCTACA
GTTTAAAGATTATCTTTTGGCTAAGAGAGAAAATGTTCCGTATAGATACTAGGAACCTTT
AGAACAGATNATGCTGAAAGACCAAGCAAAACTGAACCATGC

Last Updated on 06.12.2018 16:11