Sequences from confirmation amplicon


Confirmation sequences for line 497B06 and hit At5g37720 are shown.
Line ID 497B06
Locus At5g37720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14982964
Primers used for PCR JU36 - 8474, show primer details
   
Sequence Name 05-K025400-022-497-B06C-8409
GenBank Accession LN488199 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K025400-022-497-B06C-8409
CCGGACATGAAGCCATTTACAATTGAATATATCCTGCCGGGGCATCAGGAGTTGAAGTTG
GAACCAGGCTCCATGTTACAAATCTGGACCAGGGTGTGACAAATGAAGATATAAGGGTAT
AATCAGACTTCTATAATTTGTGCATTTGGTTTCTTAGAGGGGAGCCAGACACTAAAATGT
ATCTTCATGGGATCTAGGAACTCTTCTCTGAGATTGGGGAGGTAGAGCGTTATGCGATTC
ATTATGACAAAAATGGGCGTCCAAGTGTAAGTCCTCACAAATCTTTATCAGTCCCATTCT
CTTCATGATTTTGAAAAAGTTTTATCTTGTATGCTCTTTCTAACAGGGATTTT
   
Sequence Name 06-K025400-022-497-B06C-JU36
GenBank Accession LN488649 [GenBank]
Sequencing primer gene specific primer, JU36: 5'-CAAAATCCCTGTTAGAAAGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K025400-022-497-B06C-ju36
CTTTTTCTAACTCATGAAGAGAATGGGACTGATAAAGATTTGTGAGGACTTACACTTGGA
CGCCCATTTTTGTCATAATGAATCGCATAACGCTCTACCTCCCCAATCTCAGAGAAGAGT
TCCTAGATCCCATGAAGATACATTTTAGTGTCTGGCTCCCCTCTAAGAAACCAAATGCAC
AAATTATAGAAGTCTGATTATACCCTTATATCTTCATTTGTCACACCCTGGTCCAGATTT
GTAACATGGAGCCTGGTTCCAACTTCAACTCCTGATGCCCCGGCAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGA
TCTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
TTCAC
Primers used for PCR JU37 - 8474, show primer details
   
Sequence Name 51-K025676-022-497-B06C-8409
GenBank Accession LN507376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K025676-022-497-B06C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACCCTACGGACAGGCTATT
CAATAGAACAGAATCATTTCAGTACAAGATGTCTGTCGAACTTCAAAAACAGCGTCAATG
AAAAACAATTCCAAAGAAGTGACCACCCATATGCCTGGAAGAGGGAAACCCAGTCAAAGC
AAAGAGCTCCACCGAGTAGTCAAGAACCACCCAATGAGAAAACCAAATGAAATGGAGGAA
CAATGAGTTTGACCGACTATGCTCCGGTTCCAAAACAAATTCTTTGTACCAAGGCTATGA
TGATAAAAGAAAAACCGACGCTCATAGCATGCTGAAGAAATTCCAAATGCAGCAGGACGT
TCATCAATAACTTCATGCATGATACAGTTAAGACTGTGTAGGGGTTAGGAGGGTAAGTCA
CTATATTTACTACAACACCACACATCTTTTGAGGATTTGAAACTGTGTGACGGAAATGCA
CAACATGTCTAATTGTACTGGAAGAACAAATTTTGATCTTCATCTTACTGAAGCTCCTCA
GGTTCTAAG
   
Sequence Name 52-K025676-022-497-B06C-JU37
GenBank Accession LN507692 [GenBank]
Sequencing primer gene specific primer, JU37: 5'-TTGCGACAGTACTTAGAACCTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K025676-022-497-B06C-ju37
CTTCAGTAAGATGAAGATCAAAATTTGTTCTTCCAGTACAATTAGACATGTTGTGCATTT
CCGTCACACAGTTTCAAATCCTCAAAAGATGTGTGGTGTTGTAGTAAATATAGTGACTTA
CCCTCCTAACCCCTACACAGTCTTAACTGTATCATGCATGAAGTTATTGATGAACGTCCT
GCTGCATTTGGAATTTCTTCAGCATGCTATGAGCGTCGGTTTTTCTTTTATCATCATAGC
CTTGGTACAAAGAATTTGTTTTGGAACCGGAGCATAGTCGGTCAAACTCATTGTTCCTCC
ATTTCATTTGGTTTTCTCATTGGGTGGTTCTTGACTACTCGGTGGAGCTCTTTGCTTTGA
CTGGGTTTCCCTCTTCCAGGCATATGGGTGGTCACTTCTTTGGAATTGTTTTTCATTGAC
GCTGTTTTTGAAGTTCGACAGACATCTTGTACTGAAATGATTCTGTTCTATTGAATAGCC
TGTCCGTAGGGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11