Sequences from confirmation amplicon


Confirmation sequences for line 497E04 and hit At1g61890 are shown.
Line ID 497E04
Locus At1g61890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22868797
Primers used for PCR 19yq - 8474, show primer details
   
Sequence Name 46-K121954-0022-497-E04C-19yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 19yq: 5'-CGTTTTGTTAGAGTTCTTACCGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K121954-0022-497-E04C-19yq
ACAAACCAGTTGTCGAAACTCATTTTTTTGTTTTGTTTTTGCAGCATGTCAATTTCTGCA
ATCTCATTCATGGTCTCCGTTGGATTCAACGCAGCTGCAAGGTTGGTAACAAAACTAACA
TTATTCACTCCAAACTATAGTTATTGTTTTGCTCCTTATGGTTTACTAATAATACACTTT
TTTCAATTTATGTGTAGTGTGAGAGTAAGCAACGAGCTAGGAGCCGGAAACCCGAGAGCA
GCCGCGTTCTCCACAGTCGTGACAACGGGAGTATCATTCTTACTATCGGTTTTCGAAGCC
ATCGTGGTCTTATCGTGGCGTCACGTCATCAGCTATGCGTTTACTGATAGTATGAGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACACACGGTTA
TT
   
Sequence Name 33-K121953-0022-497-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K121953-0022-497-E04C-8409
ACTATCAGTAAACGCATAGCTGATGACGTGACGCCACGATAAGACCACGATGGCTTCGAA
AACCGATAGTAAGAATGATACTCCCGTTGTCACGACTGTGGAGAACGCGGCTGCTCTCGG
GTTTCCGGCTCCTAGCTCGTTGCTTACTCTCACACTACACATAAATTGAAAAAAGTGTAT
TATTAGTAAACCATAAGGAGCAAAACAATAACTATAGTTTGGAGTGAATAATGTTAGTTT
TGTTACCAACCTTGCAGCTGCGTTGAATCCAACGGAGACCATGAATGAGATTGCAGAAAT
TGACATGCTGCAAAAACAAAACAAAAAAATGAGTTTTCGACAACTGGTTTGTGCAAGTGT
ATGCCGTTTCTAGTCAATGGAAACGACATGCGGTAAGAACTCTAACAAAAC

Last Updated on 06.12.2018 16:11