Sequences from confirmation amplicon


Confirmation sequences for line 497F09 and hit At4g03140 are shown.
Line ID 497F09
Locus At4g03140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1392165
Primers used for PCR 14qt - 8474, show primer details
   
Sequence Name 58-K115869-0022-497-F09C-14qt
GenBank Accession LN509794 [GenBank]
Sequencing primer gene specific primer, 14qt: 5'-CCTTGAAGTTGAATGAGATGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K115869-0022-497-F09C-14qt
TCTTGCTCCATTCTTGATAATGCGTTTTTTCTATTTCATACATAGAAAAGAAAAGTTAGA
ACAAAAGAGAGGTCATTGTTAAATCTGCAGAAATGGATCAGAAGTATATATAGAGAAGGA
GCTTGACCCAATCTGAAACATGTTTGTGCTAGCTATATTGCTCCGAGATATATATATAGA
TCGGTCTTTGTCGTTTCCTAACTTATAATTTCTCGTCTTTGAAGAGAATGGTCCCTGTGT
TTGCGTCTTATATAGATATAGAGGGAACATAAAGGTGTGGTCTTTAGAGAGGGAGAGAGA
ATCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTAT
A
   
Sequence Name 17-K115867-0022-497-F09C-8409
GenBank Accession LN493998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115867-0022-497-F09C-8409
TCTCCCTCTCTAAAGACCACACCTTTATGTTCCCTCTATATCTATATAAGACGCAAACAC
AGGGACCATTCTCTTCAAAGACGAGAAATTATAAGTTAGGAAACGACAAAGACCGATCTA
TATATATATCTCGGAGCAATATAGCTAGCACAAACATGTTTCAGATTGGGTCAAGCTCCT
TCTCTATATATACTTCTGATCCATTTCTGCAGATTTAACAATGACCTCTCTTTTGTTCTA
ACTTTTCTTTTCTATGTATGAAATAGAAAAAACGCATTATTCAAGAATGTGAGCAAGAAC
TTCCTTATCAAGGGAATATCCTCATCCTCATCATCTCATTCAACTTCAAGG

Last Updated on 06.12.2018 16:11