Sequences from confirmation amplicon


Confirmation sequences for line 498B05 and hit At4g35260 are shown.
Line ID 498B05
Locus At4g35260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16775820
Primers used for PCR 20wz - 8474, show primer details
   
Sequence Name 37-K119676-0022-498-B05C-20wz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 20wz: 5'-TCTAAAACAGGCAATCTAGAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119676-0022-498-B05C-20wz
TATCTTTTTCCAAGTAAACATCGAAGACTTAAAGAACGTCATGAAAATTCATATGTATAT
AACTCTGAAACATACCATGACATCGAATTGCTCTGGTTTGGCTACAAGTTGCATACAGCA
GTTATCAACAATGATTTCATTGTAGGTGATGCTGGGATACTTTTTCGCAACCTCGCGACA
AGATTCCAAGAACAAACCATCCGCAAGTTTCATGATGTTAGCCTTATGCACCGCTGTAAC
TTTCTTCCTGTTGTTCAGGTAGGCATACTCAAAAGCATACTTTGCAATACGCTCCGAACA
GAACTTCGTGATCACCTAAGCATAGAAATAGGATCAGAACAATCACACATAACATACAAA
GACCACTTGCAAACGAAGATGATTCCACAAATACAGATTGAATCCAAAACGAGGAGCAGA
AATCGGAATTCTCACCTTCAGACTCTCAACGACACCAGGAACAACCTCGTGCTCAAGCCC
CGCGTACTCACCTTCAGTGTTCTCTCTGATGACAACGATGTCAACATTCTCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGGTTA
   
Sequence Name 13-K119674-0022-498-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K119674-0022-498-B05C-8409
GCCATTTACAATTGAATATATCCTGAGAATGTTGACATCGTTGTCATCAGAGAGAACACT
GAAGGTGAGTACGCGGGGCTTGAGCACGAGGTTGTTCCTGGTGTCGTTGAGAGTCTGAAG
GTGAGAATTCCGATTTCTGCTCCTCGTTTTGGATTCAATCTGTATTTGTGGAATCATCTT
CGTTTGCAAGTGGTCTTTGTATGTTATGTGTGATTGTTCTGATCCTATTTCTATGCTTAG
GTGATCACGAAGTTCTGTTCGGAGCGTATTGCAAAGTATGCTTTTGAGTATGCCTACCTG
AACAACAGGAAGAAAGTTACAGCGGTGCATAAGGCTAACATCATGAAACTTGCGGATGGT
TTGTTCTTGGAATCTTGTCGCGAGGTTGCGAAAAAGTATCCCAGCATCACCTACAATGAA
ATCATTGTTGATAACTGCTGTATGCAACTTGTAGCCAAACCAGAGCAATTCGATGTCATG
GTATGTTTCAGAGTTATATACATATGAATTTTCATGACGTTCTTTAAGTCTTCGATGTTT
TACTTTGAGAAAAAGATACCAGTCATCTCTCCCTAGTTTGGTATCCCTCTAGATTGCCTG
GTTTTAGA

Last Updated on 06.12.2018 16:11