Sequences from confirmation amplicon


Confirmation sequences for line 498F11 and hit At2g30830 are shown.
Line ID 498F11
Locus At2g30830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13133644
Primers used for PCR GA67 - 8474, show primer details
   
Sequence Name 48-K101501-0022-498-F11C-8409
GenBank Accession LN506461 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101501-0022-498-F11C-8409
CATTTACAATTGAATATATCCTGTCATACTATTCCCACAAATAGGTAAAATTAAAATTAC
ACAGGACTTAATACTTTGCCCACAAAAAGTTTGCACTAAAATGTAAAAAAGTTTATTTTT
TCTTTGAATATAATTTAACAAAAAGAAGAAAACAACCATACCGACAAATCACGGGTAAGT
CCCCTGCTTGTGGAACATCAGGAGCCATAACCATAGTTAAAGTATCTCTCCAATTCGCAG
CTTGAGAGCTATAGAGATCGAAATTAGTGTTATACTTAACCTTTTTAGTGATGTCACGAC
TATAGAACGTTTTCTTAACTTCTGAGTCCTGCTCATGAAACCCACGAATCCCATCTTTCA
TACTCTCCATCACCTCGAGTGGAATCCCATGGTTAATCACCTGAAAGAACCCGAACTTCT
CAACCGCAGCTCTCACTTTCGCGATCACGCTCTTTCGCGTGACCGTGGACTCGAACACGC
CACCTCCNACATCGATGGTTGGAATTTCTAACGT
   
Sequence Name 48-K101505-0022-498-F11C-GA67
GenBank Accession LN506462 [GenBank]
Sequencing primer gene specific primer, GA67: 5'-CTCACGTACCTCTGATTCAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101505-0022-498-F11C-GA67
TCTTTCTTTCACATTTTCTACCTTCTTGTCAAGAAAGAGAGACACAATGGCGGGCAACTA
TGATCGTGCTGGTGAGGTAAAAGCATTCGACCAGATGAAGATCGGCGTGAAGGGTCTTGT
CGACGCCGGAATCACAAAAGTTCCTCGCATTTTCCATCACCAAGATGTGGCCGTTACAAA
CCCTAAACCTTCCTCAACGTTAGAAATTCCAACAATCGATGTTGGAGGTGGCGTGTTCGA
GTCCACGGTCACGCGAAAGAGCGTGATCGCGAAAGTGAGAGCTGCGGTTGAGAAGTTCGG
GTTCTTTCAGGTGATTAACCATGGGATTCCACTCGAGGTGATGGAGAGTATGAAAGATGG
GATTCGTGGGTTTCATGAGCAGGACTCAGAAGTTAAGAAAACGTTCTATAGTCGTGACAT
CACTAAAAAGGTTAAGTATAACACTAATTTCGATCTCTATNGCTCTCAAGCTGCGAATTG
GAGAGATACTTTAACTA

Last Updated on 06.12.2018 16:11