Sequences from confirmation amplicon


Confirmation sequences for line 499D03 and hit At1g66280 are shown.
Line ID 499D03
Locus At1g66280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24708661
Primers used for PCR VL59 - 8474, show primer details
   
Sequence Name 31-K024724-022-499-D03C-8409
GenBank Accession LN499999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K024724-022-499-D03C-8409
TAGATTCCCGGACATGAAGCCATTTCACGAGTCAAGCTGGTGTGAAATTCTACCACGACC
TGATCGATGAGCTCCTTAAAAATGGTTATATACATATAAACTAATTAATTAAAAAAGACA
AGAAATATAGTATCATTATTTAACATTTCTATCTATTTTTACATTGTAACTAGGTATAAT
TCCGTTTGTGACTGTTTTCCATTGGGACACTCCACAAGATTTAGAAGACGAATATGGCGG
TTTCTTAAGCGAGAACATTGTGTATGTTTTTGTAAATTGAAACAAAATACATCTAATTAA
ATTTTATATGGAAATTATTAGTTTAATATCATTTTTTGCAAAAATACAGGAAAGATTTCC
GAGAATATGCAGATTATGTTTTCACTGAATACGGTGGAAA
   
Sequence Name 32-K024724-022-499-D03C-VL59
GenBank Accession LN500423 [GenBank]
Sequencing primer gene specific primer, VL59: 5'-CAGTTTTTCACTTTTCCACCGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K024724-022-499-D03C-VL59
TGAAAAATAATCTGCATATTCTCGGAAATCTTTCCTGTATTTTTGCAAAAAATGATATTA
AACTAATAATTTCCATATAAAATTTAATTAGATGTATTTTGTTTCAATTTACAAAAACAT
ACACAATGTTCTCGCTTAAGAAACCGCCATATTCGTCTTCTAAATCTTGTGGAGTGTCCC
AATGGAAAACAGTCACAAACGGAATTATACCTAGTTACAATGTAAAAATAGATAGAAATG
TTAAATAATGATACTATATTTCTTGTCTTTTTTAATTAATTAGTTTATATGTATATAACC
ATTTTTAAGGAGCTCATCGATCAGGTCGTGGTAGAATTTCACACCAGCTTGACTCGTGAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11