Sequences from confirmation amplicon


Confirmation sequences for line 500A03 and hit At5g44490 are shown.
Line ID 500A03
Locus At5g44490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17925949
Primers used for PCR 38tv - 8474, show primer details
   
Sequence Name 22-K116964-0022-500-A03C-38tv
GenBank Accession LN496278 [GenBank]
Sequencing primer gene specific primer, 38tv: 5'-GGAAAATACGATTAGGTTTCCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116964-0022-500-A03C-38tv
CATTAATAACTGGTCACTTTTTCCTCATTTTTACTTAACAGTTGTCTATAACATTTTTAA
CTAAATTGCACATTTGGATTAGTATGTTTTTCACATCTCATATCTTGGTTGTTTTTGATT
CCTATAAATGATTTACGAAGGAGCTATGCACTAACCAGTGACCTCTCATATAAAAAATTC
AATACTAAATATTATGAAAAAACCTATGTTGATCGATGCTAATCTAATCGGCCAATGCGC
TCACAAAAGAATGCATTGGCTTGTTACACCTAGATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 04-K116963-0022-500-A03C-8409
GenBank Accession LN488080 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116963-0022-500-A03C-8409
TAACAAGCCAATGCATTCTTTTGTGAGCGCATTGGCCGATTAGATTAGCATCGATCAACA
TAGGTTTTTTCATAATATTTAGTATTGAATTTTTTATATGAGAGGTCACTGGTTAGTGCA
TAGCTCCTTCGTAAATCATTTATAGGAATCAAAAACAACCAAGATATGAGATGTGAAAAA
CATACTAATCCAAATGTGCAATTTAGTTAAAAATGTTATAGACAACTGTTAAGTAAAAAT
GAGGAAAAAGTGACCAGTTATTAATGATGTTATTATATCGTTGAAAAATTTTGATACAAA
TCGGAAACCTATGGTTATTTTCAAGGAGAAAAAGAANGACTANTTACCTTTTTTTTTTCA
GTTCAAAACCGTGCATTTCC

Last Updated on 06.12.2018 16:11