Sequences from confirmation amplicon


Confirmation sequences for line 500C07 and hit At4g24020 are shown.
Line ID 500C07
Locus At4g24020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12480446
Primers used for PCR CN09 - CR3S, show primer details
   
Sequence Name 35-K030904-022-500-C07C-35St
GenBank Accession LN501711 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K030904-022-500-C07C-cr3
GTAAAAATCATAAAGTCGCCAAAAATAGTTAAGTTCTATAAAAGGAGCGGGAAGCAAGTC
AAGTTCGGTACTATAGTATCTCGAAGGGGTGAATTTTTCGGTAATCGC
   
Sequence Name 36-K030904-022-500-C07C-CN09
GenBank Accession LN502105 [GenBank]
Sequencing primer gene specific primer, CN09: 5'-GATGGAGAAGAGTCTGCAATTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K030904-022-500-C07C-CN9
AACTTGACTTGCTTTCACTCCTTTATAGAAACTTAACTCATTTTCGGCAAACTTTATGAA
TTTTACCAGAAATATCGATGTGAACTAACAATAAAGTCTCAATCACCATACCATAAATAA
ACACGAGAAATTTCAAACACTTTAGGTTCAGAATTCGCAGAAAATTAAGGAAAGAGACCT
AAATAACGTAATTGACAAATACAATTAGAAATTTTGGTTGATAGAAGTATTTTACAAATA
CAAATACATACTAAGGGTTTCCATAAATGCTCA
Primers used for PCR 59vo - CR3S, show primer details
   
Sequence Name 30-K117832-0022-500-C07C-59vo
GenBank Accession LN499899 [GenBank]
Sequencing primer gene specific primer, 59vo: 5'-TAAGAACAAAAGGTTGACCCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117832-0022-500-C07C-59vo
ACCTGAGCCAAACGTGTTGTTCGGTTGATTCTTTGAAGTATCGAAGCGCCTGAGTCATCC
TCTCTTTGATCACACAGTAGTTGTCTGTGTTCTCTGGTGGGACTAGGCTCATCAATGGAG
ACGGAAACTGGAAACTGTTATGCTTCTCTGTTGTGTTCTCAGCTGCTGATGGAGAAGAGT
CTGCAATTACCAAAAAATTCACACCTTCAAGATACTATAGTACCAAACTTGACTTGCTTC
CCACTCCTTTTATAGAACTTAACTATTTTTGGCAACTTTATGATTTTACCAGAAATATCG
ATGTGAACTAACAATAAAGTCTCAATCACCATACCATAAATAAACACGAGAAATTTCAAA
CACTTTAGGTTCAGAATTCGCAGAAAATTAAGGAAAGAGACCTAAATAACGTAATTGACA
AATACAATTAGAAATTTTGGTTGATAGAAGTATTTTACAAATACAAATACATACTAAGGG
TT
   
Sequence Name 02-K117831w-0022-500-C07C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117831w-0022-500-C07C-CR3S
CACCAAATTTCAATTGTATTTGTCATTACGTTATTTAGGTCTCTTTCCTTAATTTTCTGC
GAATTCTGAACCTAAAGTGTTTGAAATTTCTCGTGTTTATTTATGGTATGGTGATTGAGA
CTTTATTGTTAGTTCACATCGATATTTCTGGTAAAATCATAAAGTTGCCAAAAATAGTTA
AGTTCTATAAAAGGAGTGGGAAGCAAGTCAAGTTTGGTACTATAGTATCTTGAAGGTGTG
AATTTTTTGGTAATTGCAGACTCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCAT
AACAGTTTCCAGTTTCCGTCTCCATTGATGAGCCTAGTCCCACCAGAGAACACAGACAAC
TACTGTGTGATCAAAGAGAGGATGACTCAGGCGCTTCGATACTTCAAAGAATCAACCGAA
CAACACGTTTTGGCTCAGGTCTGGGCTCCTGTGAGAAAGAATGGTCGTGATTTGCTGACG
ACTTTGGGTCAACCTTATGTTCTT
Primers used for PCR 39vo - 8474, show primer details
   
Sequence Name 29-K117832-0022-500-C07C-39vo
GenBank Accession LN499464 [GenBank]
Sequencing primer gene specific primer, 39vo: 5'-TCCCTTACTTATCCTCATCGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117832-0022-500-C07C-39vo
TCTGAGCAGCCTTGCTCGCCTCTCTGGGCTTTCTCCGACGGTGGAGGAAATGGTTTTCAC
CACGCAACCTCCGGTGGCGATGATGAGAAGATCAGCTCTGTCTCCGGTGTTCCTTCTTTC
CGTCTCGCCGAGTATCCTCTCTTCCTCCCTTGTTAGTCTCGCCGACCTTTTAAGCCTTTT
TTTTTTTATTTTTATCGGATTTGCGATACATATGGATCGTATATGGGATTTTTGAATTTC
GTTATGCTAAGGGAAGGGTTTAACCTTGGATTTAGGTAGCTGCTTAAGGATTGAACATAA
ATTTTTGGTTGATTGATTGATTGATTGATTCTGCGTTTGACTTTGTTTGTCCCTTCTAAG
GAGGTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGT
ATGATGGTCAATTTGGAGAAAAAGAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAAT
GTAAATTTCCCC
   
Sequence Name 05-K117831-0022-500-C07C-oriL
GenBank Accession LN488538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117831-0022-500-C07C-oriL
GCCATTTACATTGAAAACCTCCTAAGAAGGGACAAACAAAGTCAAACGCAGAATCAATCA
ATCAATCAATCAACCAAAAATCTATGTTCAATCCTTAAGCAGCTACCTAAATCCAAGCTT
AAACCATTCCATTAGCATAACGAAATTCAAAAATCCAATATACGATCAATATGTATCGCA
ATTCCGATAAAACTAAAAAAAAAAAGGCTTAAAAGGTCGGCGAGACTAACAGGGGAGGAA
AAGAGGATACTCGGCGAGACGGAAAGAAGGAACACCGGAAACGGAGCTGATCTTCTCATC
ATCGCCCCCGGAGGTGGCGGGGGGAAAACCATTTCCTCCACCGTCGGAAAAAGCCCAAAG
AGGCAAGCAGGGCTGCTCAAAGGAAGAGGAGACAAAAATCGGAAAAATCAGGCGATTCGA
TAAGGAAAGTTAGGGGA
Primers used for PCR 04uo - 8474, show primer details
   
Sequence Name 28-K117832-0022-500-C07C-04uo
GenBank Accession LN499026 [GenBank]
Sequencing primer gene specific primer, 04uo: 5'-GAGTATCCTCTCTTCCTCCCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117832-0022-500-C07C-04uo
CTTTTTTTTTAGTTTTTCGGAATTGCGATACATATGGATCGTATATGGGATTTTTGAATT
TCGTTATGCTAAGGGAAGGGTTTAACCTTGGATTTAGGTACCTGCTTAAGGATTGAACAT
AAATTTTTGGTTGATTGATTGATTGATTGATTCTGCTTTTGACTTTGTTTGTCCCTTCTT
AGGAGGTTATTCAATTGTAAAGGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGA
GTATGATGGTCAATATGGAAAAAAAAAAAAAGTAATTCCCATTTTTTTTTCAATTCAAAA
ATGTAAATTTCCCCACCATTTTAAA
   
Sequence Name 04-K117831-0022-500-C07C-8409
GenBank Accession LN488097 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117831-0022-500-C07C-8409
ATTTACATTGAATAACCTCCTAAGAAGGGACAAACAAAGTCAAACGCAGAATCAATCAAT
CAATCAATCAACCAAAAATCTATGTTCAATCCTTAAGCAGCTACCTAAATCCAAGCTTAA
ACCATTCCATTAGCATAACGAAATTCAAAAATCCAATATACGATCAATATGTATCGCAAT
TCCGATAAAACTAAAAAAAAAAAGGCTTAAAAGGTCGGCAAGACTAACAGGGGAGGAAAA
GAGGATC
Primers used for PCR 98vo - CR3S, show primer details
   
Sequence Name 96-K117835-0022-500-C07C-98vo
GenBank Accession LN515396 [GenBank]
Sequencing primer gene specific primer, 98vo: 5'-TACTGAACATTTGGAGTCCATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K117835-0022-500-C07C-98vo
GTCACTTTCCTATCCCAGAAAACATATATGTGAGAGAGATCATCCTGTATTGATTAAGCC
CATTACCATTAGGATTAAGAACAAAAGGTTGACCCAAAGTCGTCAGCAAATCACGACCAT
TCTTTCTCACAGGAGCCCAGACCTGAGCCAAAACGTGTTGTTCGGTTGATTCTTTGAAGT
ATCGAAGCGCCTGAGTCATCCTCTCTTTGATCACACAGTAGTTGTCTGTGTTCTCTGGTG
GGACTAGGCTCATCAATGGAGACGGAAACTGGAAACTGTTATGCTTCTCTGTTGTGTTCT
CAGCTGCTGATGGAGAAGAGTCTGCAATTACCAAAAAATTCACACCTTCAAGATACTATA
GTACCAAACTTGACTTGCTTCCCACTCCTTTTATAGAACTTAACTATTTTTGGCAACTTT
ATGATTTTACCAGAAATATCGATGTGAACTAACAATAAAGTCTCAATCACCATACCATAA
ATAAACACGAGAAATTTCAAACACTTTAGGTTCAGAATTCGCAGAAAATTAAGGAAAGAG
ACCTAAATAACGTAATTGACAAATACAATTAGAAATTTTGGTTGATAGAAGTATTTTACA
AATACAAATACATACTAAGGGTT
   
Sequence Name 40-K117833w-0022-500-C07C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117833w-0022-500-C07C-CR3S
ACCAAATTTCTATTGTATTTGTCAATTACGTTATTTAGGTCTCTTTCCTTAATTTTCTGC
GAATTCTGAACCTAAAGTGTTTGAAATTTCTCGTGTTTATTTATGGTATGGTGATTGAGA
CTTTATTGTTAGTTCACATCGATATTTCTGGTAAAATCATAAAGTTGCCAAAAATAGTTA
AGTTCTATAAAAGGAGTGGGAAGCAAGTCAAGTTTGGTACTATAGTATCTTGAAGGTGTG
AATTTTTTGGTAATTGCAGACTCTTCTCCATCAGCAGCTGAGAACACAACAGAGAAGCAT
AACAGTTTCCAGTTTCCGTCTCCATTGATGAGCCTAGTCCCACCAGAGAACACAGACAAC
TACTGTGTGATCAAAGAGAGGATGACTCAGGCGCTTCGATACTTCAAAGAATCAACCGAA
CAACACGTTTTGGCTCAGGTCTGGGCTCCTGTGAGAAAGAATGGTCGTGATTTGCTGACG
ACTTTGGGTCAACCTTTTGTTCTTAATCCTAATGGTAATGGGCTTAATCAATACAGGATG
ATCTCTCTCACATATATGTTTTCTGTGGATAGTGAAAGTGACGTAGAGCTCGGACTCCCG
GGTCGAGTTTTCCGTCAGAAATTGCCTGAATGGACTCCAAATGTTCAGT

Last Updated on 06.12.2018 16:11