Sequences from confirmation amplicon


Confirmation sequences for line 500E07 and hit At1g17190 are shown.
Line ID 500E07
Locus At1g17190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5875836
Primers used for PCR ER14 - 8474, show primer details
   
Sequence Name 31-K116518-0022-500-E07C-ER14
GenBank Accession LN500310 [GenBank]
Sequencing primer gene specific primer, ER14: 5'-ATCCCATTAATGCAATGTCTAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116518-0022-500-E07C-ER14
TCTGTCTCAAGTGTCTTGAAATGTTCCAACAATTCCTTCTTCACTGCTGCATGTTCTTCG
CCCATTGTTGCCCATACCTTCCATGATGGGTCGTAAAACTGAACCAACCAAAAATCATCA
AAGTCATAAATCATTGAAAGTACGTGAGCCTAATTTTGATTATGTCATAAATCATTGAAA
AATACGTGAGCCTAATTTTGATTGTTGAACATTTTAAAAGTTGTATAGTTTTTTCTTCAT
GTTATACCAATAACTCCCAATAAATTAACTTCATCTAAAACCCTAACTAACTACAAAAAA
ATGTAGAAAAAGCGACAAAAAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
CGATTATAAT
   
Sequence Name 05-K116517-0022-500-E07C-8409
GenBank Accession LN488508 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116517-0022-500-E07C-8409
TTTTCACATTTTTTTGTAGTTAGTTAGGGTTTTAGATGAAGTTAATTTATTGGGAGTTAT
TGGTATAACATGAAGAAAAAACTATACAACTTTTAAAATGTTCAACAATCAAAATTAGGC
TCACGTATTTTTCAATGATTTATGACATAATCAAAATTAGGCTCACGTACTTTCAATGAT
TTATGACTTTGATGATTTTTGGTTGGTTCAGTTTTACGACCCATCATGGAAGGTATGGGC
AACAATGGGCGAAGAACATGCAGCAGTGAAGAAGGAATTGTTGGAACATTTCAAGACACT
TGAGACAGAGCTCGGAGACAAACCTTATTACGGTGGTGAAGTATTTGGATACCTAGACAT
TGCATTAATGGGATA

Last Updated on 06.12.2018 16:11