Sequences from confirmation amplicon


Confirmation sequences for line 500F09 and hit At4g39260 are shown.
Line ID 500F09
Locus At4g39260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18273978
Primers used for PCR AO64 - 8474, show primer details
   
Sequence Name 49-K106187-0022-500-F09C-AO64
GenBank Accession LN506848 [GenBank]
Sequencing primer gene specific primer, AO64: 5'-CGACAAAAAGAAAAGTAACGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K106187-0022-500-F09C-AO64
TTCTCAGTCAAAATTTAAACAGAAGATACTAGTATTAAATACCCAATGTCCACATTGGAT
CGGCGCAAAACTGAGTGACGATCATTTTCGATAAAATAGCACTAATTATTGGTAGGTCAA
AAGTTAAAGAACTAATCAATTTCAAACTATAGTAATTATTGATAAGAAAATCATTTGAAT
CCTTTAGCTGCTGTTCGATTAAATACATGTGTCTAACAAGATAAATTATTTGAGGGAAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 33-K106188-0022-500-F09C-8409
GenBank Accession LN501006 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K106188-0022-500-F09C-8409
ATATTATCTTGTTAGACACATGTATTTAATCGAACAGCAGCTAAAGGATTCAAATGATTT
TCTTATCAATAATTACTATAGTTTGAAATTGATTAGTTCTTTAACTTTTGACCTACCAAT
AATTAGTGCTATTTTATCGAAAATGATCGTCACTCAGTTTTGCGCCGATCCAATGTGGAC
ATTGGGTATTTAATACTAGTATCTTCTGTTTAAATTTTTGACTGAGAATATATAGACAAC
AAGATATTCATCGAATATTCCCCTTCCGTTACTTTTCTTTTTGTCG
Primers used for PCR 43vq - CR3S, show primer details
   
Sequence Name 34-K117953-0022-500-F09C-43vq
GenBank Accession LN501584 [GenBank]
Sequencing primer gene specific primer, 43vq: 5'-ATATTCCTTCCACGATTGTTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117953-0022-500-F09C-43vq
TTACGACCGCGAGAGTGGAAGATCAAGGGGATTCGGATTCGTCACCTTCAAGGACGAGAA
AGCCATGAGGGATGCGATTGAAGAGATGAACGGTAAAGAGCTCGATGGACGTGTCATCAC
CGTGAACGAGGCTCAGTCGAGAGGTAGCGGCGGTGGCGGAGGAGGCCGTGGTGGAAGCGG
TGGTGGTTACCGCAGCGGAGGCGGTGGTGGATACTCAGGAGGCGGTGGCGGCGGATACTC
AGGAGGAGGCGGTGGTGGTTACGAGAGACGTAGCGGAGGTTACGGATCTGGTGGAGGCGG
TGGTGGCCGAGGATACGGTGGTGGTGGACGCCGTGAGGGAGGTGGCTACGGAGGCGGTGA
TGGTGGAAGTTACGGAGGCGGTGGTGGCGGCTGGTAATCAAAGATAGAGTTGTTTGCGTG
CTGCTGCTCTGTTTTTGGTTTAGATTTGGTTTTGTGTCACCACTTCTGGTTTGGTTATCG
TTCGTTTGGTTTACTTTTTTGATGAAACAGTTTCGTTTAAGTCTTCTTTGTCTGGAACGA
AATGTTAATTCGCGTGTTGTTTACTAAATTTATAACGTTTCCTTTATTATTGATAGAAGT
ATTTTACAAATACAAATACATACTAAGGGTTTCTTATATGCTCAA
   
Sequence Name 10-K117952-0022-500-F09C-CR3S
GenBank Accession LN490854 [GenBank]
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117952-0022-500-F09C-CR3S
CATAAAAAGGAAACGTTATAAATTTAGTAAACAACACGCGAATTAACATCTCTTTCCAGA
CAAAGAAGACTTAAACGAAACTGTTTCATCAAAAAAGTAAACCAAACGAACGATAACCAA
ACCAGAAGTGGTGACACAAAACCAAATCTAAACCAAAAACAGAGCAGCAGCACGCAAACA
ACTCTATCTTTGATTACCAGCCGCCACCACCGCCTCCGTAACTTCCACCATCACCGCCTC
CGTAGCCACCTCCCTCACGGCGTCCACCACCACCGTATCCTCGGCCACCACCGCCTCCAC
CAGATCCGTAACCTCCGCTACGTCTCTCGTAACCACCACCGCCTCCTCCTGAGTATCCGC
CGCCACCGCCTCCTGAGTATCCACCACCGCCTCCGCTGCGGTAACCACCACCGCTTCCAC
CACGGCCTCCTCCGCCACCGCCGCTACCTCTCGACTGAGCCTCGTTCACGGTGATGACAC
GTCCATCGAGCTCTTTACCGTTCATCTCTTCAATCGCATCCCTCATGGCTTTCTCGTCCT
TGAAGGTGACGAATCCGAATCCCCTTGATCTTCCACTCTCGCGGTCGTTAATGATCTGTA
AACAAACAAAATCATACACATCAGCAAAAACAATCGTGGAAGGAATATAAT

Last Updated on 06.12.2018 16:11