Sequences from confirmation amplicon


Confirmation sequences for line 501A06 and hit At1g18720 are shown.
Line ID 501A06
Locus At1g18720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6458920
Primers used for PCR CW74 - 8474, show primer details
   
Sequence Name 73-K115869-0022-501-A06C-CW74
GenBank Accession LN512959 [GenBank]
Sequencing primer gene specific primer, CW74: 5'-TATCGGTTTGGATAAGAAATCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115869-0022-501-A06C-CW74
GGTTGGTTCCGTTTTCTTGCTGTTCGACTAGGATCTTCTCTTGCCTTAAAGGTTAGATTT
TTTTTCTCTAATTTCAATTTAGGGTTTTTGAAAAAACTTCATTTTTGGAGAAAATAATTG
CGTAGAAGCATGTTTATGAGGGTATATGACTATGTGTATCCATGTGTTGCTCTATGTGTA
TATTTATATGATGTGAGTGTGGTTCATGCATTTATTGTTCATTGTTTTCTATGCTTTGAC
TTGAAAGTTCGTTTATATCATTGGTTATGCTCGGGGAAGAAGAAATAGATTTATTTATAT
CATTAGTCCTCTCTATCTGTTGTCTATGGGTCCCTTCATTAGCTGTTAACGTTGAGACAG
CAGTACTAATATCATGTCTCTAGCTTTGAACATTCATGATTAGTAGTTATTGCAGTTTGT
TCTGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TATCCGCA
   
Sequence Name 32-K115867-0022-501-A06C-8409
GenBank Accession LN500717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115867-0022-501-A06C-8409
TTGCAGAACAAACTGCAATAACTACTAATCATGAATGTTCACATCTAGAGACATGATATT
AGTACTGCTGTCTCAACGTTAACAGCTAATGAAGGGACCCATAGACAACAGATAGAGAGG
ACTAATGATATAAATAAATCTATTTCTTCTTCCCCGAGCATAACCAATGATATAAACGAA
CTTTCAAGTCAAAGCATAGAAAACAATGAACAATAAATGCATGAACCACACTCACATCAT
ATAAATATACACATAGAGCAACACATGGATACACATAGTCATATACCCTCATAAACATGC
TTCTACGCAATTATTTTCTCCAAAAATGAAGTTTTTTCAAAAACCCTAAATTGAAATTAG
AGAAAAAAAATCTAACCTTTAAGGCAAGAGAAGATCCTAGTCGAACAGCAAGAAAACTGG
AACCAACCCAGCAAGAGAAACACATAAGAGCAGCTACAAATCCAGATTTCTTATCAAAAC
CGAT

Last Updated on 06.12.2018 16:11