Sequences from confirmation amplicon


Confirmation sequences for line 501A06 and hit At5g25550 are shown.
Line ID 501A06
Locus At5g25550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8894535
Primers used for PCR GA64 - 8474, show primer details
   
Sequence Name 02-K101423-0022-501-A06C-8409
GenBank Accession LN486965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101423-0022-501-A06C-8409
TGGCCCTTACCGATATTGCTCTGTTTCACGTTAACTCAAACCGGTTCTGTGGTACACTCC
CGGTCGGGTTTTCGCAGTTGAGTCTCCTTTTCGAACTTGATCTTAGTAATAACCGGTTCG
CTGGTAAATTCCCGGAAGTGGTAATCGGTTTACCGAAATTAAAGTATCTTGATCTCCGGT
ACAACGAGTTTGAAGGTGAATTACCAGAATCTTTGTTCGATAAAGATCTTGACGCTTTAT
TCCTGAATAGTAACCGGTTTAGAAGTAAAATCCCGGTTAACATGGGAAATTCACCGGTAT
CGGTTCTAGTTCTAGCCTCAAACCGGTTTGAAGGATGTATACCGCCGAGTTTCGGTAAAA
TGGGTAAGACTCTAAACGAGATCATTCTTATGGATAACGGTTTACAATCTTGTATACCTA
ATGACATGGGATTACTTCAAAACGTT
   
Sequence Name 42-K101426-0022-501-A06C-GA64
GenBank Accession LN504406 [GenBank]
Sequencing primer gene specific primer, GA64: 5'-AACGTTTTGAAGTAATCCCATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K101426-0022-501-A06C-GA64
CCGTTATCCTAAGAATGATCTCGTTTAGAGTCTTACCCATTTTACCGAAACTCGGCGGTA
TACATCCTTCAAACCGGTTTGAGGCTAGAACTAGAACCGATACCGGTGAATTTCCCATGT
TAACCGGGATTTTACTTCTAAACCGGTTACTATTCAGGAATAAAGCGTCAAGATCTTTAT
CGAACAAAGATTCTGGTAATTCACCTTCAAACTCGTTGTACCGGAGATCAAGATACTTTA
ATTTCGGTAAACCGATTACCACTTCCGGGAATTTACCAGCGAACCGGTTATTACTAAGAT
CAAGTTCGAAAAGGAGACTCAACTGCGAAAACCCGACCGGGAGTGTACCACAGAACCGGT
TTGAGTTAACGTGAAACAGAGCAATATCGGTTAAGAGGCCAAGCTCTTCAGGGAGATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCANAAATGTAGA

Last Updated on 06.12.2018 16:11