Sequences from confirmation amplicon


Confirmation sequences for line 501B04 and hit At1g55440 are shown.
Line ID 501B04
Locus At1g55440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20701811
Primers used for PCR CC72 - 8474, show primer details
   
Sequence Name 36-K105706-0022-501-B04C-CC72
GenBank Accession LN502226 [GenBank]
Sequencing primer gene specific primer, CC72: 5'-TGGTTGTATGGAGTGTGATTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105706-0022-501-B04C-CC72
CCTCCCAATACATAAACACCCACTTACCCTCCACCCCTTCCCGATTGGTGGGATCTCCAG
GTTCGATTTTAATACAGTTGCTGCCAAAGACATATTCAAATGTAAAGCTTGCTACCGATT
AAGTTGTGGTTTCGTGTACAAATGCGGTGAGGAATATTGTGAGTTTCAAATAGACGTCAG
ATGCGCTTCCCTTCCAGATCCTTTAATTCATGGCTGTCATCCACATGATCTCTTCTTCAA
CTTGACCCATGGTTATTGCATTGGGTGTAGACCACGAACCGACTTGACCAAGGCACGTAT
GCTCAGCTCACATGGGCCGGGCCTTTGTTCATCCTATGCAGTAGAATGTATTAAGTGCGA
CGACTCCTTTTTGGGTATGACATGTGCTAGTTTTCCTTGTGAGGCCTACTACAAGCACGA
TAGGCATCTACTCACACTTTGCTACGGTGACGAAAAAGATACAACAATGGCTTGAAAGAG
TAAATGGCTTCATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATATCCG
   
Sequence Name 52-K105707-0022-501-B04C-8409
GenBank Accession LN507819 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K105707-0022-501-B04C-8409
TCGGACTGACCATTTACTGAAGCCATTTACTCTTTCAGCCATTGTTGTATCTTTTTCGTC
ACCGTAGCAAAGTGTGAGTAGATGCCTATCGTGCTTGTAGTAGGCCTCACAAGGAAAACT
AGCACATGTCATACCCAAAAAGGAGTCGTCGCACTTAATACATTCTACTGCATAGGATGA
ACAAAGGCCCGGCCCATGTGAGCTGAGCATACGTGCCTTGGTCAAGTCGGTTCGTGGTCT
ACACCCAATGCAATAACCATGGGTCAAGTTGAAGAAGAGATCATGTGGATGACAGCCATG
AATTAAAGGATCTGGAAGGGAAGCGCATCTGACGTCTATTTGAAACTCACAATATTCCTC
ACCGCATTTGTACACGAAACCACAACTTAATCGGTAGCAAGCTTTACATTTGAATATGTC
TTTGGCAGCAACTGTATTAAAATCGAACCTGGAGATCCCACCAATCGGGAAGGGGTGGAG
GGTAAGTGGGTGTTTATGTATTGGGAGGTATTTTTTGCGAGGAAGAGAAGCACATGCTTC
GTCGAGTACAAAATCACACTCCATACAACC
Primers used for PCR 54wp - 8474, show primer details
   
Sequence Name 24-K118804-0022-501-B04C-54wp
GenBank Accession LN497204 [GenBank]
Sequencing primer gene specific primer, 54wp: 5'-TGTCTCCCACAACTTTATCACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118804-0022-501-B04C-54wp
GAAAAGCAGAATGAATGACAACGTCTCTATAAGGACTCTACTTATATAACACCTAAAGAT
ATAATAACAGCAAAGTGATCTTGATAGACTCCAAAACCAAAACGAGAACAGAATTTTATC
ATTTTCATTTTCATCAACACAAAAGAAAAAAAAGAAAACAAAAATAAAATTTAACACCAA
AAGATTAAACCCTAAGAAACGTGTATTCGACGAAAGCCCTGAAACACATAAGATGCGCTG
ATCACAATGTCTTGGCTACCTTCAATTGAGACGTCCAAGGCCAAGACCTTTTCCCATACA
ATTAAGAGTGCAATACCATTTGTATCCACGTATCATCTTGAATGCCCAAGCCTGTGCACA
ATGAAGATTACATTCAGAGCAAATCAGCCGAGAGTTACCATCATTGTGAACAATTTCAAC
TTCATCACCATCATCACCTATTTCGAATATATGGCCGGGCTTCATGTACATATCTTTTCC
AAGTAAACAATCGATATGTAAAGTGATCATACAAGAGTCACATGTATAAAACCAGTCATC
TGCATTTAATTCTGATTCACATATCTCACACCAAAGCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 05-K118803-0022-501-B04C-8409
GenBank Accession LN488568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118803-0022-501-B04C-8409
TTTACATTGAATATATCCTGCTTTGGTGTGAGATATGTGAATCAGAATTAAATGCAGATG
ACTGGTTTTATACATGTGACTCTTGTATGATCACTTTACATATCGATTGTTTACTTGGAA
AAGATATGTACATGAAGCCCGGCCATATATTCGAAATAGGTGATGATGGTGATGAAGTTG
AAATTGTTCACAATGATGGTAACTCTCGGCTGATTTGCTCTGAATGTAATCTTCATTGTG
CACAGGCTTGGGCATTCAAGATGATACGTGGATACAAATGGTATTGCACTCTTAATTGTA
TGGGAAAAGGTCTTGGCCTTGGACGTCTCAATTGAAGGTAGCCAAGACATTGTGATCAGC
GCATCTTATGTGTTTCAGGGCTTTCGTCGAATACACGTTTCTTAGGGTTTAATCTTTTGG
TGTTAAATTTTATTTTTGTTTTCTTTTTTTTCTTTTGTGTTGATGAAAATGAAAATGATA
AAATTCTGTTCTCGTTTTGGTTTTGGAGTCTATCAAGATCACTTTGCTGTTATTATATCT
TTAGGTGTTATATAAGTAGAGTCCTTATAGAGACGTTGTCATTCATTCTTGCTTTTTCCA
CACCACATACTTTACTATAGTCCAAGTCATGGCGGATGTGATAAAGTTGTGGGGAGACA
Primers used for PCR AP36 - 8474, show primer details
   
Sequence Name 25-K118804-0022-501-B04C-AP36
GenBank Accession LN497675 [GenBank]
Sequencing primer gene specific primer, AP36: 5'-CCAAGACCTTTTCCCATACAATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118804-0022-501-B04C-AP36
GCCTGTGCACAATGAAGATTACATTCAGAGCAAATCAGCCGAGAGTTACCATCATTGTGA
ACAATTTCAACTTCATCACCATCATCACCTATTTCGAATATATGGCCGGGCTTCATGTAC
ATATCTTTTCCAAGTAAACAATCGATATGTAAAGTGATCATACAAGAGTCACATGTATAA
AACCAGTCATCTGCATTTAATTCTGATTCACATATCTCACACCAAAGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAAGATTATTATAAT
   
Sequence Name 06-K118803-0022-501-B04C-oriL
GenBank Accession LN489021 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118803-0022-501-B04C-oriL
TCATTGAATATATCCTGCTTTGGTGTGAGATATGTGAATCAGAATTAAATGCAGATGACT
GGTTTTATACATGTGACTCTTGTATGATCACTTTACATATCGATTGTTTACTTGGAAAAG
ATATGTACATGAAGCCCGGCCATATATTCGAAATAGGTGATGATGGTGATGAAGTTGAAA
TTGTTCACAATGATGGTAACTCTCGGCTGATTTGCTCTGAATGTAATCTTCATTGTGCAC
AGGCTTGGGCATTCAAGATGATACGTGGATACAAATGGTATTGCACTCTTAATTGTATGG
GAAAAGGTCTTGG

Last Updated on 06.12.2018 16:11