Sequences from confirmation amplicon


Confirmation sequences for line 502A09 and hit At1g29680 are shown.
Line ID 502A09
Locus At1g29680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10378296
Primers used for PCR JY03 - 8474, show primer details
   
Sequence Name 29-K024286-022-502-A09C-JY03
GenBank Accession LN499126 [GenBank]
Sequencing primer gene specific primer, JY03: 5'-ACACTTACCATGTTGCTTCCAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024286-022-502-A09C-JY03
CAGCCATCGGATTGATCCACTCCGGTTCAGCAATCTCCGCTCTCGTGTGTTTCAAATCAT
CTGTCGAAATGTTGTACTTCTCATCTCTTCTCTTCACCAACTCTGGCCTTAGTACCCCTT
GCCCCACCCCCTGAGGTGACATCATTAGTTCGGGTGCACCCATTGGTAACTTGTCACCTA
CAGAAACACACATAAATAACCAATGATTGGTCGGATCAAACTAAACCGGACAAAATCGAA
CCCACTTCTAGAATTTCTATGATTCTGATTAATGTTTGAATTAGTTAACCTCTGAGTCCG
GATAACTAATTGTTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCACGTCCCAT
   
Sequence Name 30-K024286-022-502-A09C-8409
GenBank Accession LN499562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024286-022-502-A09C-8409
GATTTCCCGGACATGAAGCCATTTAAACAATTAGTTATCCGGACTCAGAGGTTAACTAAT
TCAAACATTAATCAGAATCATAGAAATTCTAGAAGTGGGTTCGATTTTGTCCGGTTTAGT
TTGATCCGACCAATCATTGGTTATTTATGTGTGTTTCTGTAGGTGACAAGTTACCAATGG
GTGCACCCGAACTAATGATGTCACCTCAGGGGGTGGGGCAAGGGGTACTAAGGCCAGAGT
TGGTGAAGAGAAGAGATGAGAAGTACAACATTTCGACAGATGATTTGAAACACACGAGAG
CGGAGATTGCTGAACCGGAGTGGATCAATCCGATGGCTGACTACTGGAAGCAACATGGTA
AGTG

Last Updated on 06.12.2018 16:11