Sequences from confirmation amplicon


Confirmation sequences for line 502D03 and hit At1g75380 are shown.
Line ID 502D03
Locus At1g75380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28282802
Primers used for PCR 21vh - 8474, show primer details
   
Sequence Name 57-K117914-0022-502-D03C-21vh
GenBank Accession LN509536 [GenBank]
Sequencing primer gene specific primer, 21vh: 5'-GATTTAAGTTTCCAAGGCTGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K117914-0022-502-D03C-21vh
ATAACTGGGCAAATATGCCTCGTGAACTCTTTTGGTGACTCTAACAAGTCTAACCTGTAG
AATAGTTCAGAAAATTTTCATGAAACACAATCTTCACGAGACAAGGATATTTAAATAAAA
GAGCAGAAACTGAAACCACGAAACATACTTCGTAACCCATCTTGTCCACCATTTCCTTCA
CCACTTGATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 17-K117913-0022-502-D03C-8409
GenBank Accession LN494045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117913-0022-502-D03C-8409
TCATTGTATCAGTGGTGAAGGAAATGGTGGACAAGATGGGTTACGAAGTATGTTTCGTGG
TTTCAGTTTCTGCTCTTTTATTTAATATCCTTGTCTCGTGAAGATTGTGTTTCATGAAAA
TTTTCTGAACTATTCTACAGGTTAGACTTGTTAGAGTCACCAAAAGAGTTCACGAGGCAT
ATTTTGCCCAGTTATTCCTTTCAAAGGTCTGCAACTGGTCTCTTATATACAACTTACATT
CAGCCTTGGAAACTTAAATCAAAAGAANGAGTNNTTTCCCCTTTTTTTTCTCTTCAAAAA
TGTTGACGTCCGCCCCGTTAAAAAAAAA

Last Updated on 06.12.2018 16:11