Genotyping and primer details


Genotyping and primer details for 502D03 - At1g75380
Line ID 502D03
Locus At1g75380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28282802 of line 502D03 Chr1:28282802
   
Insertion allele amplicon 8474 - 21vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21vh: 5'-GATTTAAGTTTCCAAGGCTGAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28282545
   
wt amplicon (over InsPos) 35vu - 21vh
wt amplicon start Chr1:28282545
wt amplicon end Chr1:28283462
wt amplicon size 918 bp
Locus specific primer 21vh: 5'-GATTTAAGTTTCCAAGGCTGAATG-3'
2nd locus specific primer 35vu: 5'-TGTGCAACAAGAGTATCAAGTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11